An ÄKTApurifier System (GE Healthcare Life Sciences, Uppsala, Sweden) equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) was used. Eighty micrograms of MET‐ECD‐His, 40 μg HGF, or a mixture of both was incubated in PBS with 0.1 mmol/L sucrose octasulfate sodium salt (Toronto Research Chemicals, Toronto, Ontario, Canada) for 10 minutes at room temperature, and then subjected to size‐exclusion chromatography. Samples were eluted with PBS containing 0.1 mmol/L sucrose octasulfate sodium salt at a flow rate of 0.5 mL/min at 4°C. Fractions of 0.3 mL were collected and analyzed using absorbance at 280 nm. Eluted fractions were subjected to SDS‐PAGE using a 5%‐20% polyacrylamide gradient gel and stained using a Rapid CBB Stain Kit (Nacalai Tesque, Kyoto, Japan).
Sucrose octasulfate sodium salt
Manufactured by LGC
Sourced in Canada
Sucrose octasulfate sodium salt is a chemical compound used in various laboratory applications. It functions as a sulfated polysaccharide, known for its ability to interact with proteins and other biomolecules. The product is typically used in research and analysis within the scientific community.
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Lab products found in correlation
Kta purifier system, by GE Healthcare (1 mentions)
Superdex 200 increase 10 300 gl column, by GE Healthcare (1 mentions)
Trichloroacetic acid, by Fujifilm (1 mentions)
Pepsin, by Fujifilm (1 mentions)
Sodium carbonate, by Fujifilm (1 mentions)
Casein, by Fujifilm (1 mentions)
Isoflurane inhalation solution, by Pfizer (1 mentions)
2 protocols using sucrose octasulfate sodium salt
1
Size-Exclusion Chromatography of MET-ECD-His and HGF
2
Sucrose Octasulfate Oral Administration
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Sucrose octasulfate sodium salt (Toronto Research Chemicals, Inc., Toronto, ON, Canada) was dissolved in distilled water for all experiments and was administered orally at a volume of 2.5 mL/kg of body weight. The following drugs and reagents were also utilized: isoflurane inhalation solution (Pfizer, New York, NY, USA); 10% sucralfate-containing suspension (ULCERLMIN® Oral Suspension 10%; Chugai Pharmaceutical Co. Ltd., Tokyo, Japan); pepsin (1:10,000, from porcine stomach mucosa), casein, trichloroacetic acid, sodium carbonate, and Folin’s reagent (all purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan); and 9-aminoacridine (9-AA; Thermo Fisher Scientific K.K., Yokohama, Japan).
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