In order to obtain semi-IPN hydrogels with antibacterial properties, three types of chitosan were used: commercial chitosan (CC, Sigma-Aldrich St Louis, MO, USA, ≥75% deacetylation degree, Mn = 2.056 × 105 g/mol), chitosan synthesized in previous works from commercial chitin via a deacetylation process (CCH, 77% deacetylation degree, Mn = 4.7291 × 105 g/mol [52 (link)]) and mineral-enriched chitosan extracted in previous works from shrimp shells (SHC, 76% deacetylation degree, Mn = 9.058 × 103 g/mol, crystallinity 59.42% on (020) plane [52 (link)]). Vinyl benzyl trimethylammonium chloride (Sigma-Aldrich, 99%, VBTAC) was used as a monomer to generate quaternary ammonium pendant groups. The synthesis of semi-IPN hydrogels was performed following the initiation process with 4,4′-azobis-4-cyanovaleric acid (Sigma-Aldrich ≥ 98%, ACVA) in the presence of N,N′-methylenebisacrylamide (Sigma Aldrich ≥ 98%, MBA) as a crosslinking agent. The listed compounds were used without any purification. Acetic acid (Sigma-Aldrich, 99%) and pure demineralized water were also used to solubilize chitosan.
Vbtac
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2 protocols using vbtac
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Antibacterial Chitosan-based Semi-IPN Hydrogels
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Functional Organosilanic Monomers for Film Preparation
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For films preparation, the functional organosilanic monomers [3-(2-trimethoxysilyl) propylmethacrylate (MAPTES, 98%, Sigma Aldrich) and trimethoxysilane (MPTES, 98%, Sigma Aldrich)], ammonium hydroxide -catalyst (NH4OH, 25 %, ChimReactiv), Ethanol (EtOH, 99.6%, Fisher Scientific), hydrochloric acid (HCl, 99.6%, Fisher Scientific) and distilled water were used as such. The lipopolysaccharide template from Pseudomonas Aeruginosa 10 (LPS with 500,000 endotoxin units/mg, Sigma-Aldrich) was used in the form of lyophilized powder (as received).
For microparticles prepration, kaolin (K, ACROS Organics), vinyltrimethoxysilane (VTMS, 99%, Fluka), vinylbenzyl trimethylammonium chloride (VBTAC, 98%, Sigma Aldrich), 2,2′-Azobis (2methylpropionitrile) (AIBN, 98%, Sigma Aldrich) radical initiator, ethanol (EtOH, 99.6%, Fisher Scientific), isopropanol (S.C. Reagents COM S.R.L) and dimethylforamide (DMF, 99.8%, Sigma-Aldrich) were used as such without any further purification.
For microparticles prepration, kaolin (K, ACROS Organics), vinyltrimethoxysilane (VTMS, 99%, Fluka), vinylbenzyl trimethylammonium chloride (VBTAC, 98%, Sigma Aldrich), 2,2′-Azobis (2methylpropionitrile) (AIBN, 98%, Sigma Aldrich) radical initiator, ethanol (EtOH, 99.6%, Fisher Scientific), isopropanol (S.C. Reagents COM S.R.L) and dimethylforamide (DMF, 99.8%, Sigma-Aldrich) were used as such without any further purification.
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