Methyl salicylate

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, Germany

Methyl salicylate is a chemical compound used in laboratory settings. It is a colorless liquid with a characteristic wintergreen odor. Methyl salicylate's core function is as a solvent and chemical intermediate in various applications.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using methyl salicylate

Quantification of Mammary Gland Ductal Density

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

After fixation, whole-mounted mammary glands were processed through an alcohol series, defatted with toluene, stained with carmine alum, dehydrated in an alcohol and xylene series, and preserved in k-pax heat-sealed bags (Thermo Fisher Scientific) with methyl salicylate (Acros Organics, Morris Plains, NJ) [51 (link)]. Two digital images of whole-mount mammary glands (one from the left; one from the right third pectoral glands) were obtained using an AxioImager dissection microscope (Carl Zeiss Microscopy, Jena, Germany) at ×30 magnification and a Zeiss high-resolution color camera. To evaluate mammary gland morphology, ZEN software (Carl Zeiss Microscopy) was used to measure ductal density [51 (link)]. A 13 × 16 grid (180 crosshairs, 0.3 mm apart) was placed on each image, and the fraction of crosshairs that fell on ducts was quantified and averaged for the two images for each sample.

LaPlante C.D., Bansal R., Dunphy K.A., Jerry D.J, & Vandenberg L.N. (2018). Oxybenzone Alters Mammary Gland Morphology in Mice Exposed During Pregnancy and Lactation. Journal of the Endocrine Society, 2(8), 903-921.

+ Open protocol

+ Expand

Salicylate-Based Analytical Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following reagents were purchased from Acros Organics: hydrochloric acid, sodium tetraborate, sodium salicylate, methylsalicylate, salicylamide, sodium hydrocarbonate, citric acid, sodium chloride, aminoacids, titanium(IV) tetraethoxyde, and tetraethyl orthosilicate. All the reagents were of analytical grade, titanium(IV) tetraethoxyde was of technical grade.
Stock solutions of salicylate and salicylamide were prepared with doubly distilled water. Stock solution of methylsalicylate was prepared with ethanol. Only freshly prepared solutions of these substances were used.
Trinder reagent was prepared as in [13 (link)]: 4.0 g of iron (III) nitrate nonahydrate and 5.0 g of trichloroacetic acid were dissolved in 100.0 mL of doubly distilled water.

Morosanova M.A, & Morosanova E.I. (2015). Silica-titania xerogel for solid phase spectrophotometric determination of salicylate and its derivatives in biological liquids and pharmaceuticals. Chemistry Central Journal, 9, 64.

+ Open protocol

+ Expand

Curcumin-Methylsalicylate Simulant Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methylsalicylate (99%) and curcumin (98 + %, a mixture of dimethoxycurcumin and bisdimethoxycurcumin) were purchased from Acros Organics, UK. The simulant dosing solution ("CMX") was prepared on the day of each experiment by gradually adding 250 mg curcumin to 25 mL Methylsalicylate (giving a final curcumin concentration of 10 mg mL -1 ) in a lightproof glass container. The mixture was then sonicated (Nickel Electro Ltd. model SW3H, Fisher Scientific, UK) for 15 min under lightproof conditions and stored at room temperature for a maximum of 1 h before use.
Isopropyl alcohol (high-performance liquid chromatography [HPLC] grade), acetonitrile (HPLC grade), methanol (HPLC grade), and glacial acetic acid (HPLC grade) were purchased from Scientific Laboratory Supplies Ltd., Nottinghamshire, UK. Ultra-pure water (>18.2 M ) for LC analysis was filtered from the municipal supply via a MilliQ Integral 3 (Millipore, MA, USA).
Volunteers were provided with plain black swimwear (bikinis for female volunteers, swimming briefs for males) and plain black unisex outer garments (100% cotton long-sleeved t-shirts and leisure trousers), all purchased from Primark, Hampshire, UK.

Chilcott R.P., Mitchell H, & Matar H. (2019). Optimization of Nonambulant Mass Casualty Decontamination Protocols as Part of an Initial or Specialist Operational Response to Chemical Incidents. Prehospital emergency care, 23(1).

+ Open protocol

+ Expand

Molecular Characterization of Tumor Angiogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

2HF (purity ∼99%), DMBA, horseradish peroxidase (HRP)-conjugated anti-mouse, and anti-rabbit secondary antibodies, and MTT were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies against pAKT (S⁴⁷³), PCNA, CD31, Ki67, CDK1, Bcl2, maspin, survivin, Bax, vimentin, and E-cadherin antibodies were purchased from Santa Cruz Biotechnology (Columbus, OH) and Cell Signaling Technologies (Danvers, MA). The avidin/biotin complex detection kit was procured from Vector (Burlingame, CA). Mayer’s hematoxylin, methyl salicylate, and Permount were purchased from Fisher Scientific (Hanover Park, IL). The source of RLIP antibodies was the same as previously described (16 (link)).

Singhal S.S., Horne D., Singhal J., Vonderfecht S., Salgia R, & Awasthi S. (2019). Synergistic efficacy of RLIP inhibition and 2'-Hydroxyflavanone against DMBA-induced mammary carcinogenesis in SENCAR mice. Molecular carcinogenesis, 58(8), 1438-1449.

+ Open protocol

+ Expand

Confocal Imaging of Cy3-Labeled Brain Structures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brains were dissected and fixed in 4% paraformaldehyde, 0.25% glutaraldehyde, and 2% saturated picric acid diluted in 0.1 mol l⁻¹ phosphate buffered saline (PBS) at 4 °C overnight. Following rinses in PBS (4 × 15 min) brains were incubated in Cy3-conjugated streptavidin (1:1000) in PBS containing 0.3% Triton X-100 (PBT) at 4 °C for 3 days. Following rinses in PBT (2 × 20 min) and PBS (3 × 20 min) brains were dehydrated in an ascending ethanol series (30%, 50%, 70%, 90%, 95%, 100%, 15 min each) and cleared in a 1:1 mixture of 100% ethanol and methyl salicylate (Merck, Darmstadt, Germany) for 15 min and in pure methyl salicylate for 1 h. Finally, they were mounted in Permount (Fisher Scientific, Pittsburgh, PA) between two coverslips. Samples were scanned with a confocal laser scanning microscope (Leica, TCS SP5, Leica Microsystems, Wetzlar, Germany) with a 20 × immersion objective (HC PL APO 20 ×/0.75 Imm Corr CS2, Leica). A diode pumped solid state laser (561 nm) was used to excite Cy3. Scanning frequency was 400 Hz and the voxel size was 0.54 × 0.54 × 2.0 µm³.

Hensgen R., Zittrell F., Pfeiffer K, & Homberg U. (2022). Performance of polarization-sensitive neurons of the locust central complex at different degrees of polarization. Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology, 208(3), 387-403.

+ Open protocol

+ Expand

Whole Mount Mammary Gland Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following fixation, whole mount mammary glands were washed, dehydrated through an alcohol series and defatted with toluene (Sigma-Aldrich, St. Louis MO). The glands were then stained overnight with carmine alum (Sigma-Aldrich), dehydrated through a series of alcohols and xylene, and then placed in k-pax heat-sealed bags (Fisher) with methyl salicylate (Fisher) to preserve them.

Kolla S., Pokharel A, & Vandenberg L.N. (2017). The mouse mammary gland as a sentinel organ: distinguishing ‘control’ populations with diverse environmental histories. Environmental Health, 16, 25.

+ Open protocol

+ Expand

Histological Analysis of Regenerating Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following amputation, tissues were fixed overnight in 10% neutral buffered formalin, and decalcified in 10% EDTA (Sigma). The next day, regenerates were dehydrated in a series of ethanol dilutions and cleared overnight in methyl salicylate (Fisher). Tissues were next infiltrated with molten paraffin at 80°C and embedded in paraffin blocks. 15 μm sections were obtained and placed on Superfrost Plus (VWR) slides. Following collection, sections were deparaffinised in xylene for 3 minutes, then rehydrated using a series of diluted ethyl alcohol washes with a final incubation in distilled water for 3 minutes. Tissues were next stained in 1% alcian blue (Sigma)/3% glacial acetic acid for 30 minutes, followed by a 0.1% Fast Red Nuclear Dye (Sigma) stain. Finally, sections were dehydrated in a series of ethyl alcohol washes, and incubated in xylene for 3 minutes. Slides were imaged using a dissecting microscope equipped with a Nikon DS-U2 camera (Tokyo, Japan) and NIS Elements Software (Mississaugua, Ontario, Canada).

Walker S.E., Nottrodt R., Maddalena L., Carter C., Spencer G.E, & Carlone R.L. (2018). Retinoid X receptor α downregulation is required for tail and caudal spinal cord regeneration in the adult newt. Neural Regeneration Research, 13(6), 1036-1045.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!