Molecular weight marker, reagents and nitrocellulose membranes for SDS‐PAGE were purchased from Bio‐Rad (Mississauga, ON). SignalFirePlus ECL reagent (catalogue #12630), protease/phosphatase inhibitor cocktail (catalogue #5872), and SDS loading buffer/dithiothreitol (catalogue #7722) were from Cell Signaling Technologies (Danvers, MA). Horseradish peroxidase–conjugated donkey anti‐rabbit and goat anti‐mouse IgG secondary antibodies were from Jackson ImmunoResearch Laboratories (West Grove, PA). Antibodies against BACE (#5606), ERK (#4695), phospho ERK (threonine 202/tyrosine 204; #9101), p38 (#9212), phospho p38 (threonine 180/tyrosine 182; #9211), JNK (#9252), phospho JNK (threonine 183/tyrosine 185; #4671), GAPDH and VINCULIN were purchased from Cell Signaling (Danvers, MA).
Nitrocellulose membranes for sds page
Manufactured by Bio-Rad
Nitrocellulose membranes are a type of porous membrane commonly used in SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) applications. These membranes provide a solid support for the separation and transfer of proteins from polyacrylamide gels to a stable matrix, enabling further analysis and detection techniques.
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Lab products found in correlation
Gapdh, by Cell Signaling Technology (2 mentions)
Horseradish peroxidase conjugated donkey anti rabbit and goat anti mouse igg secondary antibodies, by Jackson ImmunoResearch (1 mentions)
Vinculin, by Cell Signaling Technology (1 mentions)
Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions)
Signalfire plus ecl reagent, by Cell Signaling Technology (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Goat anti mouse igg secondary antibody, by Jackson ImmunoResearch (1 mentions)
Vinculin, by Merck Group (1 mentions)
P ampk, by Cell Signaling Technology (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated donkey anti rabbit, by Jackson ImmunoResearch (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
Pakt s473, by Cell Signaling Technology (1 mentions)
2 protocols using nitrocellulose membranes for sds page
1
Western Blot Analysis of MAPK Signaling
2
Western Blot Antibody Source Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Horseradish peroxidase‐conjugated donkey anti‐rabbit and goat anti‐mouse IgG secondary antibodies were from Jackson ImmunoResearch Laboratories (Westgrove,PA). Molecular weight marker, reagents and nitrocellulose membranes for SDS‐PAGE were acquired from Bio‐Rad (Mississauga, ON). Western lightning Plus‐ELC (PerkinElmer, 105001EA). Antibodies for GAPDH (#2118S), BACE1 (#5606P), AMPK (#2793S), pAMPK (#2531S), AKT (#4685S), pAKT S473 (#4058S), pAKT Thr172 (#2531S), ERK 1/2 (#4695S), pERK 1/2 (#9101S), p38 (#9212S), p‐p38 (#9211S), JNK (#9252S), pJNK (#4671S), GSK‐3β (#9315S) and pGSK‐3β (#5558S) were from Cell Signaling (Danvers, MA, USA) and Vinculin (#05‐386) was from Milipore. All other sources are listed throughout the text.
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