Luc+ B16F10 cells were purchased from ATCC (ATCC CRL-6475-LUC2). They were cultured in Dulbecco’s modified Eagle’s media containing 10% FBS, 1% penicillin–streptomycin, and, if Luc+, with 10 μg/mL Blasticidin. Cells were split at a 1:5 ratio every 3 days when approximately 80% confluent. For in vivo experiments, they were injected at passage 3 when 50% confluent. Cells tested negative for mycoplasma using Lonza Mycolert Mycoplasma Detection Kit prior to experiments.
Mycolert mycoplasma detection kit
Manufactured by Lonza
The Mycolert Mycoplasma Detection Kit is a laboratory equipment product designed for the detection of mycoplasma contamination in cell cultures. The kit provides a reliable and efficient method for identifying the presence of mycoplasma in samples.
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Lab products found in correlation
2 protocols using mycolert mycoplasma detection kit
1
Culturing and Injecting Luciferase-Expressing B16F10 Cells
2
EG7 Cell Culture Conditions
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EG7 cells were purchased from ATCC. They were cultured in RPMI media containing 10% FBS, 1% penicillin–streptomycin, and 0.05 mM 2-mercaptoethanol. Cell media was supplemented every 2 days and upon confluency (1e6 cells/mL), cells were split by dilution to a seeding density of 100,000 cells/mL. Cells tested negative for mycoplasma using Lonza Mycolert Mycoplasma Detection Kit prior to experiments.
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