Log In Sign up
The largest database of trusted experimental protocols

Ti perfect focus system

Manufactured by Nikon
Visit Supplier

The Nikon Ti Perfect Focus system is a microscope focusing system designed to maintain focus during long-term live-cell imaging. It uses an infrared beam to continuously monitor and adjust the focus, compensating for drift and other environmental factors that can affect the sample.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Nis elements analysis software, by Nikon (2 mentions) Fluorodish culture plates, by World Precision Instruments (2 mentions)

Spelling variants of this product

Perfect Focus System (283 citations) Perfect Focus (28 citations) Ti-E Perfect Focus System (3 citations) Eclipse Ti-U with perfect-focus system (2 citations)

2 protocols using ti perfect focus system

1

Live-cell confocal imaging of HEK293T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were captured using a Nikon A1R resonant scanning multispectral confocal microscope (Nikon Instruments, Inc. Melville, NY), equipped with a live cell chamber (37°C with humidified 5% CO2), Nikon Ti Perfect Focus system, and NIS-Elements analysis software (Nikon). 48 h prior to live-cell imaging, HEK293T cells plated onto 0.1% gelatin-coated FluoroDish™ culture plates (35 mm, World Precision Instruments Inc.) were rinsed twice with DMEM (GIBCO) to remove residual lipids and respective treatments were added. Prior to imaging, culture media was refreshed. Time-lapse videos were obtained at 100x magnification (1.45 NA oil objective) at 5–20 s intervals over a 5 min duration.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
+ Open protocol
+ Expand
2

Live-cell confocal imaging of HEK293T cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were captured using a Nikon A1R resonant scanning multispectral confocal microscope (Nikon Instruments, Inc. Melville, NY), equipped with a live cell chamber (37°C with humidified 5% CO2), Nikon Ti Perfect Focus system, and NIS-Elements analysis software (Nikon). 48 h prior to live-cell imaging, HEK293T cells plated onto 0.1% gelatin-coated FluoroDish™ culture plates (35 mm, World Precision Instruments Inc.) were rinsed twice with DMEM (GIBCO) to remove residual lipids and respective treatments were added. Prior to imaging, culture media was refreshed. Time-lapse videos were obtained at 100x magnification (1.45 NA oil objective) at 5–20 s intervals over a 5 min duration.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!