Northern blot experiments were carried out as described in [85 (link)]. pBAD2-gfp served as a DNA template for the amplification of a 286 bp fragment possessing a T7 RNA promotor. Based on the described fragment, an in vitro produced and DIG-labeled gfp RNA probe (Roche, Mannheim, Germany) was prepared for the detection of gfp transcripts.
Dig labeled gfp rna probe
Manufactured by Roche
The DIG-labeled gfp RNA probe is a laboratory equipment product that serves as a tool for detecting and visualizing the expression of the green fluorescent protein (GFP) gene in various experimental systems. The probe is labeled with digoxigenin (DIG), a hapten molecule, which allows for the specific detection of the GFP RNA transcript through various hybridization techniques.
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Lab products found in correlation
2 protocols using dig labeled gfp rna probe
1
Northern Blot for GFP Transcript Detection
2
Northern Blot Detection of GFP
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Northern blot experiments were carried out as described in [83] (link). pBAD2-gfp served as a DNA template for the amplification of a 286 bp fragment possessing a T7 RNA promotor. Based on the described fragment, an in vitro produced and DIG-labeled gfp RNA probe (Roche, Mannheim, Germany) was prepared for the detection of gfp transcripts.
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