Gossypol (GOS), valproic acid sodium salt (VPA), Tween 80, suberoylanilide hydroxamic acid (SAHA), tubacin, 3-methyladenine (3-MA), propidium iodide (PI), dimethyl sulfoxide (DMSO), Hoechst 33342, and MG132 were purchased from Sigma-Aldrich (St. Louis, MO, USA). GOS was dissolved in dimethyl sulfoxide (DMSO). The final concentration of DMSO never exceeded 0.2%, which had no cytotoxicity in this study (data not shown). WST-1 assay kit was obtained from Roche (Penzberg, Germany). RNase A, Dulbecco's modified Eagle's medium (DMEM), RPMI-1640, fetal bovine serum (FBS), penicillin and streptomycin were purchased from Invitrogen (Carlsbad, CA, USA). Polyvinylidene difluoride (PVDF) membranes (Hybond-P) were purchased from GE Healthcare Life Sciences (Piscataway, NJ, USA). The antibodies against cyclin A2 (#4656), Bcl-2 (#2870), Bax (#2772), Bim (#2933), PARP(#9532), cleaved caspase-3 (#9664), cleaved caspase-8 (#9496), cleaved caspase-9 (#7237), phospho(p)-Akt (Thr308: #2965), Akt (#2920), FOXO3a (#2497), p-FOXO3a (Ser253: #9466), acetyl-histone H3 (Lys9: #9649), acetyl-histone H4 (Lys16: #8804), histone H3 (#9717), LC3B (#3868), and β-tubulin (#2128) were obtained from Cell Signaling Technology (Danvers, MA, USA). The antibody against survivin (sc-10811) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Polyvinylidene difluoride pvdf membranes hybond p
Manufactured by GE Healthcare
Sourced in United States
Polyvinylidene difluoride (PVDF) membranes (Hybond-P) are a type of lab equipment used for various biological and analytical applications. These membranes are made of a highly durable and chemically resistant polymer material, PVDF. They are designed to provide a stable and consistent platform for various techniques, such as Western blotting, immunodetection, and protein analysis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Tubacin, by Merck Group (2 mentions)
β tubulin, by Cell Signaling Technology (2 mentions)
Hoechst 33342, by Merck Group (2 mentions)
Sqstm1 p62, by Cell Signaling Technology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Suberoylanilide hydroxamic acid, by Merck Group (1 mentions)
Valproic acid sodium salt, by Merck Group (1 mentions)
Acetyl histone h4, by Cell Signaling Technology (1 mentions)
Gossypol, by Merck Group (1 mentions)
Wst 1 assay kit, by Roche (1 mentions)
Cyclin a2, by Cell Signaling Technology (1 mentions)
Histone h3, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Acetyl histone h3, by Cell Signaling Technology (1 mentions)
Cleaved caspase 8, by Cell Signaling Technology (1 mentions)
Foxo3a, by Cell Signaling Technology (1 mentions)
P foxo3a, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
3 protocols using polyvinylidene difluoride pvdf membranes hybond p
1
Multimodal Regulation of Cell Pathways
2
Cucurbitacin E Mediated Autophagy Regulation
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Cucurbitacin E was obtained from Shanghai Shunbo Chempharm Co (Shanghai, China). Chloroquine (CQ), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS), polyformaldehyde were from Sigma-Aldrich (St. Louis, MO, USA). WST-1 was from Roche (Penzberg, Germany). Lipofectamine RNAiMAX, Dulbecco's modified Eagle's medium (DMEM), penicillin, streptomycin, L-glutamine, and fetal bovine serum (FBS) were from Gibco/ Invitrogen (Carlsbad, CA, USA). Polyvinylidene difluoride (PVDF) membranes (Hybond-P) were obtained from GE Healthcare Life Sciences (Piscataway, NJ, USA). Antibodies against p-p70S6KT389, p70S6K, p-4E-BP1T37/46, 4E-BP1, p-S6S235/236, S6, p62/SQSTM1, LC3B, ATG5, Beclin 1, p-AKTS473, p-AktT308, Akt, p-AMPKαT172, AMPKα, p-AMPKβ1S108, AMPKβ1, p-ULK1S758, p-ULK1S555, ULK1, p-RaptorS792, Raptor, β-tubulin and HRP-conjugated second antibody were obtained from Cell Signaling Technology (Danvers, MA, USA). The antibody against LAMP2 was obtained from Abcam (Cambridge, MA, USA).
3
Acetylation and Autophagy Assays
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Temozolomide, tubacin, Hoechst33342 and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), antibiotics and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA, USA). Polyvinylidene difluoride (PVDF) membranes (Hybond-P) and enhanced chemiluminescence (ECL) kit were purchased from GE Healthcare Life Sciences (Piscataway, NJ, USA). Antibodies against acetyl- a-tubulin, a-tubulin, LC3B, p62/ SQSTM1, and HRP-conjugated sheep anti-rabbit IgG were all obtained from Cell Signaling Technology(Danvers, MA, USA). LAMP2 mouse monoclonal IgG was purchased from Santa Cruz.
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