Rat anti mouse cd117 2b8

Embryos were prepared as previously described (Yokomizo et al., 2012 (link)). A Zeiss LSM 710 AxioObserver inverted confocal microscope with ZEN 2011 software was used to acquire Z-projections and single optical projections. Images were processed using Fiji software (Schindelin et al., 2012 (link)). 3-dimensional reconstructions were produced using Volocity software (PerkinElmer). The following primary antibodies were used; rat anti-mouse CD31 (Mec 13.3, BD Pharmingen, San Diego, CA), rat anti-mouse CD117 (2B8, eBioscience, San Diego, CA), chicken anti-GFP (polyclonal, Thermo Fisher Scientific, Waltham, MA), rabbit anti-mouse Prox1 (AngioBio, San Diego, CA) and rabbit anti-human/mouse Runx (EPR3099, Abcam, Cambridge, MA). Secondary antibodies used were goat anti-rat Alexa Fluor 647 (Invitrogen, Carlsbad, CA), goat-anti rat Alexa Fluor 555 (Abcam), donkey anti-rat Alexa Fluor 555 (Abcam), goat anti-chicken Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA) and goat anti-rabbit Alexa Fluor 488 (Invitrogen).

Embryos were prepared as previously described (Yokomizo et al., 2012 (link)). A Zeiss LSM 710 AxioObserver inverted confocal microscope with ZEN 2011 software was used to acquire Z-projections and single optical projections. Images were processed using Fiji software (Schindelin et al., 2012 (link)). 3-dimensional reconstructions were produced using Volocity software (PerkinElmer). The following primary antibodies were used; rat anti-mouse CD31 (Mec 13.3 , BD Pharmingen, San Diego, CA), rat anti-mouse CD117 (2B8, eBioscience, San Diego, CA), chicken anti-GFP (polyclonal, Thermo Fisher Scientific, Waltham, MA) and rabbit anti-human/mouse Runx (EPR3099, Abcam, Cambridge, MA). Secondary antibodies used were goat anti-rat Alexa Fluor 647 (Invitrogen, Carlsbad, CA), goat-anti rat Alexa Fluor 555 (Abcam), donkey anti-rat Alexa Fluor 555 (Abcam), goat anti-chicken Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA) and goat anti-rabbit Alexa Fluor 488 (Invitrogen).