The adipose SVCs and splenocytes were incubated for 10 minutes with CD16/32 antibody (FC block) (eBioscience) and then stained with other fluorochrome-conjugated antibodies for 20 minutes at 4℃. All cells were stained with antibodies against CD45-PE/Cy7 (eBioscience), CD4-BV500 (BD Biosciences), CD8-APC/Cy7 (Biolegend), CD19-PE (Biolegend), NK1.1-BV421 (Biolegend), NKp46-PE/Dazzle594 (Biolegend), CD11b-BV786 (Biolegend), CD11c-APC (Biolegend), CD3-BV711 (Biolegend), and F4/80-BV650 (Biolegend). 7AAD (Invitrogen) was used for live/dead staining. The stained cells were washed and analyzed with an LSRFortessa flow cytometer (BD Biosciences). NK cells were defined as CD45+ F4/80− CD19− CD3− NK1.1+ in lymphocyte gating. The NKp46+ and GFP+ cells were then gated. ATMs were defined as CD45+ CD19− CD3− NK1.1− F4/80+ CD11b+. Total cell numbers were counted with a hemocytometer and normalized according to the tissue weight (cells/g). Flow cytometric data were analyzed by using the FlowJo software (Flow Jo).
Cd11b bv786
Manufactured by BioLegend
Sourced in United States
CD11b-BV786 is a fluorochrome-conjugated antibody that binds to the CD11b antigen, also known as the integrin αM subunit or Mac-1 alpha chain. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils, and is involved in cellular adhesion and phagocytosis.
Automatically generated - may contain errors
Lab products found in correlation
Cd8 apc cy7, by BioLegend (1 mentions)
Cd3 bv711, by BioLegend (1 mentions)
Nk1.1 bv421, by BioLegend (1 mentions)
F4 80 bv650, by BioLegend (1 mentions)
Cd45 pe cy7, by Thermo Fisher Scientific (1 mentions)
Cd19 pe, by BioLegend (1 mentions)
Cd11c apc, by BioLegend (1 mentions)
Lsrfortessa, by BD (1 mentions)
7 aad, by Thermo Fisher Scientific (1 mentions)
Facsdiva 8, by BD (1 mentions)
Cd8 fitc, by BD (1 mentions)
Cd14 apc, by BD (1 mentions)
Cd16 pe, by BD (1 mentions)
Cd19 apc, by BD (1 mentions)
Cd69 pe cy7, by BioLegend (1 mentions)
Live dead staining, by Thermo Fisher Scientific (1 mentions)
Cd206 fitc, by BD (1 mentions)
Lyric, by BD (1 mentions)
Cd4 bv421, by BD (1 mentions)
2 protocols using cd11b bv786
1
Comprehensive Immune Cell Profiling
2
Multiparameter Flow Cytometry Characterization
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The characterization of differentiated MDM was performed via flow cytometry with the following mAbs: CD11b BV 786 (Biolegend, San Diego, CA, USA; clone IRCF44), CD14 APC (BD Biosciences, Franklin Lakes, NJ, USA; clone M5E2), CD206 FITC (BD Biosciences, clone 19.2), HLA-DR BV711 APC (BD Biosciences, clone G46-6), CD16 PE (BD Biosciences, clone 3G8), CD4 BV 421 (BD Biosciences, clone RPA-T4), CD8FITC (BD Biosciences, clone RPA-T8), CD19 APC (BD Biosciences, cloneHIB19) and CD69PECy7 (Biolegend, clone G10F5). Live dead staining (ThermoFisher, Waltham, MA, USA) was performed as a control for vitality. Labelled cells were fixed in PBS−/− 1× 1% formalin. A minimum of 50.000 events were acquired for each sample using a BD Lyric (BD Biosciences) and analyzed by FACS Diva 8.0.1 (BD Biosciences).
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