Total RNA was extracted from cells using an RNA purification kit (K0731, Thermo Fisher Scientific). The Prime Script RT Reagent Kit (RR036B, TaKaRa) was used for the reverse transcription of RNA into cDNA. qPCR was performed using a SYBR Premix Ex Taq Kit (11 199ES03, Yeasen) on a CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, California, USA). PCR primers are listed in online supplemental table 2 .
Sybr premix ex taq kit
Manufactured by Yeasen
Sourced in United States, China
The SYBR Premix Ex Taq kit is a reagent used in real-time PCR (polymerase chain reaction) experiments. It contains a DNA polymerase enzyme, SYBR Green I dye, and other necessary components required for the amplification and detection of DNA sequences.
Automatically generated - may contain errors
Lab products found in correlation
Primescript rt reagent kit, by Takara Bio (1 mentions)
Rna purification kit, by Thermo Fisher Scientific (1 mentions)
Cfx96 real time pcr detection system, by Bio-Rad (1 mentions)
Tnf α elisa kit, by MultiSciences Biotech (1 mentions)
Lipopolysaccharide lps from escherichia coli 0111 b4, by Merck Group (1 mentions)
Primescript rt kit, by Yeasen (1 mentions)
Ab7500 fast real time pcr system, by Thermo Fisher Scientific (1 mentions)
Rt master mix kit, by Yeasen (1 mentions)
Total rna kit, by Tiangen Biotech (1 mentions)
3 protocols using sybr premix ex taq kit
1
Quantitative Real-Time PCR Protocol
2
Asiatic Acid Modulates Inflammatory Responses
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Asiatic acid (CAS: 464-92-6) was purchased from Feiyu Biotechnology Co., LTD (Nantong, China). Lipopolysaccharide (LPS, from Escherichia coli 0111:B4) was purchased from Sigma-Aldrich (St. Louis, USA). Methanol was purchased from Sinopharm (Shanghai, China). IL-10, IL-6, and TNF-α ELISA kits were obtained from Multisciences (Hangzhou, China). MPO kits were purchased from Elabscience (Wuhan, China). PrimeScript RT kit and SYBR Premix Ex Taq kit were purchased from YEASEN (Nanjing, China). Primers for PCR analysis were purchased from BGI (Beijing, China), with sequences shown in Table S2 . γ-CD (CAS: 17465-86-0) and HPCD (CAS: 128446-35-5) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China).
3
Rat Gene Expression Quantification
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Rat mRNA was isolated from cells or tissues with a Total RNA kit (Tiangen, Shanghai, China) and reverse transcribed with an RT Master Mix kit (Yeasen, Shanghai, China). qRT-PCR was performed with an SYBR Premix Ex Taq kit (Yeasen) on an AB 7500 Fast Real-Time PCR System (Applied Biosystems). Rat β-actin was used as an internal control. The sequences of primers are listed in Table S1 . The fold changes in mRNA expression levels were normalized to β-actin using the ΔΔCt method.
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