Mcf 7 cells

All the cell lines used in this study were obtained from Holding Company for Biological Products and Vaccines VACSERA, Egypt; MCF-7 cells (Human breast adenocarcinoma), HepG2 (human hepatocellular carcinoma), Caco-2 (colon carcinoma), A549 (human lung adenocarcinoma), PANC-1 (human pancreatic cancer), Vero cells (derived from normal kidney cells).
The RPMI 1640 medium (Lonza, Switzerland) was used to maintain all cell lines. The media also contained 10% fetal bovine serum (Gibco, USA), 1% penicillin, and 1% streptomycin (Sigma Aldrich, USA). In a humidified cell incubator with a 5% Carbon dioxide environment, the cells were kept at 37 °C. The study was examined and approved by the Ethics committee in the Faculty of Pharmacy-October University for Modern Sciences and Arts (MSA) with ethics approval number (BP2/Ec2/2021PD).

MCF7 cells were purchased from the Tissue Culture Core at Baylor College of Medicine. BT474, T47D, and THP1 cells were purchased from ATCC; Jurkat (J32) cells were a gift from Dr. Andras Heczey; hMSC cells were purchased from Lonza (PT-2501). MCF7 cells were cultured in DMEM with 10% FBS; T47D cells were cultured in RPMI with 10% FBS; BT474 cells were cultured in DMEM with 10% FBS and 15 μg/ml insulin; THP1 and Jurkat cells were cultured in RPMI with 10% FBS and 1% L-glutamine; hMSCs were cultured using the MSCGM BulletKit from Lonza. All cell lines were cultured with 1% penicillin–streptomycin (ThermoFisher Scientific) and maintained at 37 °C in a humidified incubator with 5% CO₂. All cell lines were confirmed to be free of mycoplasma contamination by DNA staining with Hoechst (ThermoFisher Scientific) or Syto 82 (ThermoFisher Scientific). DMEM, RPMI, and L-glutamine were purchased from ThermoFisher Scientific, and FBS and bovine insulin were purchased from Sigma. All cell lines were authenticated by the Cell Line Authentication Testing Division of Labcorp.