The MG MPs (20 mg) and the free MG (20 mg) were put into small vials containing 2 mL of water, AGJ and AIJ, respectively and sealed, and then all the vials were put in the water bath of 37 °C, 40 rpm/min. After 48 h, the samples were taken out and centrifuged at 8000 rpm for 10 min, and then filtered through a 0.22 μm membrane filter. The supernatant obtained was used for HPLC detection to determine MG concentration. The HPLC detection was implemented by a Waters 1525-2489 high performance liquid chromatograph (Waters Corporation, Milford, MA, USA) consisting of a pump (Waters 1525 binary) and UV detector (Waters 2489 Tunable Absorbance Detector), which was equipped with a Diamonsil C18 reverse-phase column (4.6 × 250 mm, 5 μm, DIKMA, Beijing, China). The mobile phase, composed of acetonitrile and 0.1% phosphoric acid, was delivered at 1 mL/min. The drug was detected at 325 nm and the injection volume was 10 μL. The linear regression equation y = 13,866,374.4100x + 50,262.5833 (R2 = 0.9998) was obtained with concentration of MG standard solution as abscissa and the absorbency as the y-coordinate.
Waters 1525 binary
Manufactured by Waters Corporation
Sourced in United States
The Waters 1525 binary is a high-performance liquid chromatography (HPLC) pump designed for analytical and preparative applications. It features two independent solvent delivery systems that can be operated in a binary gradient mode, allowing for the precise control and mixing of two different solvents. The 1525 binary provides accurate and reproducible flow rates, making it a reliable and versatile tool for a wide range of chromatographic separations.
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Lab products found in correlation
Diamonsil c18 reverse phase column, by Dikma Technologies (2 mentions)
Waters 2489 tunable absorbance detector, by Waters Corporation (2 mentions)
Waters 2707, by Waters Corporation (1 mentions)
Waters 2489, by Waters Corporation (1 mentions)
717 plus autosampler, by Waters Corporation (1 mentions)
Uv vis 2487 detector, by Waters Corporation (1 mentions)
5 protocols using waters 1525 binary
1
Quantification of Miglitol in Aqueous Media
2
HPLC Quantification of Hydroxykynurenine
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HK was quantified by a Waters 1525-2489 high performance liquid chromatograph (Waters Corporation, Milford, MA, USA) consisting of a pump (Waters 1525 binary) and UV detector (Waters 2489 Tunable Absorbance Detector), which was equipped with a Diamonsil C18 reverse-phase column (4.6 × 250 mm, 5 μm, DIKMA, Beijing, China). A methanol (55%), acetonitrile (20%), and water (25%) mixture was used as mobile phase. Other parameters of HPLC detection were as follows: A wavelength was 294 nm, the flow rate of the mobile phase was 1 mL/min, and the injection volume was 10 μL.
A standard solution of HK was prepared as follows: HK (10 mg) was accurately weighed and dissolved in 10 mL methanol solution, and then the solution was diluted appropriately to obtain solutions of 0.5, 0.25, 0.125, 0.0625, 0.03125, 0.015625, 0.007813, and 0.003906 mg/mL. Using the concentration of HK standard solution as the abscissa and the absorbency as the y-coordinate, the linear chart was constructed, and the regression equation was Y = 11326308.72X − 8679 (R2 = 0.9998). The retention time of the HK was about 10–11 min.
A standard solution of HK was prepared as follows: HK (10 mg) was accurately weighed and dissolved in 10 mL methanol solution, and then the solution was diluted appropriately to obtain solutions of 0.5, 0.25, 0.125, 0.0625, 0.03125, 0.015625, 0.007813, and 0.003906 mg/mL. Using the concentration of HK standard solution as the abscissa and the absorbency as the y-coordinate, the linear chart was constructed, and the regression equation was Y = 11326308.72X − 8679 (R2 = 0.9998). The retention time of the HK was about 10–11 min.
3
Quantification of Fatty Acids via HPLC
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For FA quantification a Breeze™2 HPLC equipped with a Symmetry® C18 5 µm 4′6 × 75 mm analytical column, UV/ Visible detector Waters 2489 set at 307 nm (the maximum absorption of FA), Waters 2707 autosampler and Waters 1525 binary HPLC pump was used. A gradient mode reversed-phase high-performance liquid chromatography (RP-HPLC) (Table S1 †) was employed. Mobile phases used were a 0.001 wt% acetic acid solution in Milli-Q water (solvent A) and acetonitrile (solvent B) at 25 °C. Measurements were conducted at a flow rate of 1 mL min -1 , injecting a volume of 50 µL, and 20 min chromatogram plotting.
4
Precise Quantification of MC-LR by HPLC-UV
5
Soil PCP Extraction and Quantification
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Soil extractable PCP was obtained following the method of Khodadoust [28] . The soil samples (2 g, dry weight) were weighed in polycarbonate centrifuge tubes and suspended in 40 mL water:ethanol (50:50, v/v). The tubes were sealed, placed on an orbital shaker at 180 rpm for 1 h, and then centrifuged at 3,000 g for 15 min. The supernatants were passed through a 0.45 μm filter and the precipitate was separated as humic acid, fulvic acid and humin fractions using 0.5 mol L -1 NaOH and HCl, respectively [26] . The earthworm-accumulated PCP was extracted according to Parrish et al. [29] , and the bound PCP residues were extracted from the three fractions as described by Nieman et al. [26] and Scelza et al. [27] .
Soil PCP was quantified using high-performance liquid chromatography (HPLC, Waters 1525/2487, USA), supplemented with a Waters 1525 binary pump, an analytical reversed-phase column (5 μm Pinnacle II C18, 4.6 mm (i.d.) and 25 cm long, Waters, USA) and a Waters 2487 dual λ absorbance UV/vis detector. The mobile phase consisted of 80% methanol and 20% water (1% acetic acid) and was pumped at a rate of 1.0 mL min -1 . Measurements were taken under isocratic conditions at 40±1°C at a wavelength of 220 nm. Soil PCP concentration was determined according to the standard curves from five external PCP standards.
Soil PCP was quantified using high-performance liquid chromatography (HPLC, Waters 1525/2487, USA), supplemented with a Waters 1525 binary pump, an analytical reversed-phase column (5 μm Pinnacle II C18, 4.6 mm (i.d.) and 25 cm long, Waters, USA) and a Waters 2487 dual λ absorbance UV/vis detector. The mobile phase consisted of 80% methanol and 20% water (1% acetic acid) and was pumped at a rate of 1.0 mL min -1 . Measurements were taken under isocratic conditions at 40±1°C at a wavelength of 220 nm. Soil PCP concentration was determined according to the standard curves from five external PCP standards.
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