A sample of approximately 10 mg of left ventricular tissues was homogenized in Tris HCl buffer with SDS and centrifuged. About 70 µg of supernatant protein were subjected to SDS gel electrophoresis. Anti- phospho p44/42 MAP kinase (9101) and anti GAPDH (14C10) antibodies were purchased from Cell Signaling. Following immunoblotting the gel bands were subjected to densitometric scanning.
Anti phospho p44 42 map kinase 9101
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho p44/42 MAP kinase (9101) is a primary antibody that detects the phosphorylated forms of p44/42 mitogen-activated protein kinases (MAPK). It recognizes the dually phosphorylated, active forms of p44 (ERK1) and p42 (ERK2) MAPK.
Automatically generated - may contain errors
2 protocols using anti phospho p44 42 map kinase 9101
1
Western Blot Analysis of Cardiac Proteins
2
Western Blot Analysis of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested and protein prepared as described previously [33 (link)]. The primary antibodies used were anti-EGFR, anti-pEGFR, anti-AKT, anti-pAKT, anti-PARP1/2 (sc-7150), anti-p53 (sc-126), anti-p21 (sc-756), and anti-α-tubulin (sc-8035) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-phospho-p44/42 MAP kinase (#9101) (Cell Signaling, Boston, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!