Cardiomyocyte supernatant was analyzed by ELISA kits for interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) (BD Biosciences, San Jose, CA) according to the manufacturer’s instructions. Cardiac and lung tissue was crushed in liquid nitrogen, and equal weights of powdered tissues (~ 50 mg) were dissolved in 500 µl of lysis buffer (10 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, and 2 mM each of EDTA and EGTA), and subjected to sonication on ice. Protein concentration was determined by the BioRad protein assay reagent (Hercules, CA). Equal amounts of proteins (250–500 µg) were loaded into respective ELISA wells for the assessment of IL-6 and IL-1β (Invitrogen, Carlsbad, CA). Serum was analyzed using a Bio-Plex Pro Mouse Cytokine Th17 Panel A -6-Plex kit (BioRad).
Il 6 and il 1β
Manufactured by Thermo Fisher Scientific
The IL-6 and IL-1β products are laboratory assays used to detect and measure the levels of the cytokines interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) in biological samples. These cytokines play important roles in immune and inflammatory responses. The products provide a standardized and quantitative method for researchers to analyze IL-6 and IL-1β concentrations in their experiments.
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2 protocols using il 6 and il 1β
1
Cytokine Profiling in Cardiomyocyte and Tissue
2
Cytokine Profiling in Cardiomyocyte and Tissue
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Cardiomyocyte supernatant was analyzed by ELISA kits for interleukin (IL)-6 and tumor necrosis factor-α (TNF-a) (BD Biosciences, San Jose, CA) according to the manufacturer's instructions. Cardiac and lung tissue was crushed in liquid nitrogen, and equal weights of powdered tissues (~50 mg) were dissolved in 500 µl of lysis buffer (10 mM Hepes, pH 7.4, 5 mM MgCl 2 , 1 mM DTT, 1% Triton X-100, and 2 mM each of EDTA and EGTA), and subjected to sonication on ice. Protein concentration was determined by the BioRad protein assay reagent (Hercules, CA). Equal amounts of proteins (250-500 µg) were loaded into respective ELISA wells for the assessment of IL-6 and IL-1β (Invitrogen, Carlsbad, CA). Serum was analyzed using a Bio-Plex Pro Mouse Cytokine Th17 Panel A -6-Plex kit (BioRad).
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