Cell migration was measured by wound-healing assay by the IncuCyte HD system (IncuCyte ZOOM, Essen BioScience, USA). Cells were seeded in 96-well plates at the density of 6×104 cells per well. Wound was made through confluent monolayer cells with a pin block and cells were washed with 1×PBS, cultured in DMEM medium with 10% fetal bovine serum. Photographs of cells were imaged using the IncuCyte HD system at 4-hour intervals from 2 separate regions per well using a 10x objective. Values from 2 regions of each well were pooled and averaged across all 3 replicates.
Incucyte hd system incucyte zoom
Manufactured by Sartorius
Sourced in United States
The IncuCyte HD system is an automated live-cell analysis platform that enables real-time monitoring and quantification of cellular processes. It provides continuous, non-invasive imaging and analysis of cells in their native environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using incucyte hd system incucyte zoom
1
Wound-Healing Assay for Cell Migration
2
Scratch Wound Migration Assay
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Scratch wounding migration assay was detected by the IncuCyte HD system (IncuCyte ZOOM, Essen BioScience, USA). NCI-N87 and AGS cells were grown in 96-well culture plates until the formation of a monolayer. Cell layers were scraped with a pin block and then incubated at 37°C. Photographs were captured at set time points by IncuCyte HD system. The migration distance was measured at each time point in 3 independent samples.
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