G2505b 2 dye scanner

This was performed following protocols from Agilent. Briefly, 600 ng of each labeled cRNA was fragmented and then mixed with hybridization buffer using the Agilent gene expression hybridization kit. These were applied to an ovine 8 X 15 K array slide (Agilent 019921), containing 8 arrays with 15,208 oligomers with a length of 60 bases and hybridized at 65°C for 17 h at 10 rpm. The arrays were washed, dried, stabilized, and scanned with an Agilent G2505B 2 dye scanner at the Interdisciplinary Center for Biotechnology Research at the University of Florida. Features were extracted with Agilent Feature extraction 9.1 software. Microarray data have been deposited in the NCBI Gene Expression Omnibus under accession number GSE99497.

This was performed following protocols from Agilent. Briefly, 600 ng of each labeled cRNA was fragmented and then mixed with hybridization buffer using the Agilent gene expression hybridization kit. These were applied to sheep 8 × 15 K array slides (Agilent 019921), containing 8 arrays with 15,208 oligomers with a length of 60 bases and hybridized at 65°C for 17 h at 10 rpm. A total of 3 slides (24 arrays) were employed per each brain region (six gestational ages times four replicates per gestational age). The arrays were washed, dried, stabilized, and scanned with an Agilent G2505B 2 dye scanner at the Interdisciplinary Center for Biotechnology Research at the University of Florida. Features were extracted with Agilent Feature extraction 9.1 software.