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Mueller hinton lab agar

Manufactured by Biomaxima
Sourced in China

Mueller Hinton Lab-Agar is a microbiological growth medium used for the cultivation and isolation of various bacterial species. It is formulated to support the growth of a wide range of microorganisms and is commonly used in antimicrobial susceptibility testing, such as the Kirby-Bauer disk diffusion method.

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2 protocols using mueller hinton lab agar

1

Chitosan-Alginate Nanocomposite Synthesis

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The following chemical reagents were used to produce the nanocomposites: chitosan (high molecular weight: 310,000–375,000 Da, degree of deacetylation >75%) from shrimp shells, (Sigma-Aldrich, Saint Louis, MI, USA), sodium alginate (Sigma-Aldrich), acetic acid (Chempur, 99.5%), glycerine (99.5%, Chempur), deionized water, extra virgin olive oil, turmeric, ethanol 96% p.a. grade.
The following microbial strains were used for microbiological testing: Escherichia coli (DSMZ 1116), Aspergillus fumigatus (LOCK 042600), Penicillium expansum (LOCK 0535). The growth mediums used in the experiment: Mueller Hinton Lab-Agar (BIOMAXIMA), DRBC Lab-Agar (BIOMAXIMA).
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2

Antifungal Activity Assay Protocol

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Pluronic F-127, RPMI-1640 medium with the addition of l-glutamine and without
sodium bicarbonate, PBS (phosphate-buffered saline), propidium iodide, and amphotericin B
were purchased from Sigma-Aldrich (USA). Nile red was obtained from Santa Cruz
Biotechnology (USA). Fluconazole and verapamil hydrochloride were obtained from Pol-Aura
(Poland). Sterile, purified water was purchased from Polpharma (Poland).
3-morpholinopropanesulfonic acid (MOPS) was purchased from J&K Scientific (China).
Mueller Hinton LAB-AGAR with glucose and methylene blue were both purchased from Biomaxima
(Poland). Sabouraud dextrose agar with chloramphenicol was purchased from Liofilchem
(Italy). Sabouraud dextrose liquid medium was purchased from Oxoid (Great Britain).
Tryptic soy broth was bought from Biomaxima (Poland). Zymo Research—YeaStar RNA Kit
was obtained from TK Biotech (Poland). The primers were ordered from Genomed (Poland). The
TranScriba Kit and the RT HS-PCR Mix Probe were supplied by A&A Biotechnology
(Poland).
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