The pure samples of the following compounds: nonanal, 2,3-butanedione (diacetyl), acetic acid, 3-methylbutanal, butanoic acid, 2-methylpropanoic acid, 3-methylbutanoic acid, pentanoic acid, octanoic acid, and hexanoic acid were purchased from Merck (Darmstadt, Germany). (E)-2-nonenal, 2-nonanone, phenyl acetaldehyde, δ-decalactone, 2-methoxy-4-vinylphenol, 4,5-epoxy-(E)-2-decanal, (E,E)-2,4-decadienal, (Z)-2-nonenal, methional, and 4-hydroxy-2,5-dimethyl-3 (2H)-furanone were from Sigma–Aldrich (Taufkirchen, Germany). 1-Octen-3-one was from Symrise (Holzminden, Germany). The following labelled compounds were synthesized according to the literature cited; [2H2]-butanoic acid [19 (link)]; 3-[2H2]-methylbutanal [11 (link)]; [2H2-5]-2-acetyl-1-pyrroline [20 (link)]; [13C4]-2,3-butanedione [21 (link)]; [2H]-(E,E)-2,4-decadienal [22 ]; [13C2]-acetic acid [22 ]; [2H2]-3-methylbutanoic acid [23 (link)]; [2H3]-2-methoxyphenol [24 (link)]; [2H5]-phenyl acetaldehyde [25 (link)]; [13C2]-4-hydroxy-2,5-dimethyl-3(2H)-furanone [26 (link)]; [2H2]-δ-decalactone [27 (link)]; [2H2]-methional [27 (link)]. Lastly, [2H3]-hexanoic acid was from Cambridge Isotope Laboratories (Euriso-top GmbH, Saarbrucken, Germany).
4 hydroxy 2 5 dimethyl 3 2h furanone
Manufactured by Merck Group
Sourced in Germany
4-hydroxy-2,5-dimethyl-3(2H)-furanone is a chemical compound with the molecular formula C6H8O2. It is a white, crystalline solid that is soluble in organic solvents. The compound serves as a key intermediate in the synthesis of various organic compounds and is used in research and development applications.
Automatically generated - may contain errors
Lab products found in correlation
Phenylacetaldehyde, by Merck Group (3 mentions)
2 methoxyphenol, by Merck Group (3 mentions)
Acetic acid, by Merck Group (3 mentions)
Butanoic acid, by Merck Group (3 mentions)
3 methylbutanoic acid, by Merck Group (3 mentions)
Methional, by Merck Group (2 mentions)
2 methoxy 4 vinylphenol, by Merck Group (2 mentions)
Linalool, by Merck Group (2 mentions)
2 methylbutanal, by Merck Group (2 mentions)
3 hydroxy 4 5 dimethyl 2 5h furanone, by Merck Group (2 mentions)
2 3 pentanedione, by Merck Group (2 mentions)
3 methylbutanal, by Merck Group (2 mentions)
5 ethyl 4 hydroxy 2 methyl 3 2h furanone, by Merck Group (2 mentions)
Octanoic acid, by Merck Group (1 mentions)
Hexanoic acid, by Merck Group (1 mentions)
Pentanoic acid, by Merck Group (1 mentions)
Nonanal, by Merck Group (1 mentions)
2 nonanone, by Merck Group (1 mentions)
δ decalactone, by Merck Group (1 mentions)
E 2 nonenal, by Merck Group (1 mentions)
7 protocols using 4 hydroxy 2 5 dimethyl 3 2h furanone
1
Compound Acquisition for Flavor Analysis
2
Quantification of Aroma Compounds by GC-MS
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The following reference compounds: acetic acid, acetoin, 2-methylbutanal, 2-methylpyrazine, 2,5-dimethylpyrazine, 2-furanmethanethiol, 2-acetyl-1-pyrroline, benzaldehyde, 1-octen-3-one, octanal, linalool, 2-acetylthiazole, 2-acetylpyridine, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, 2-methoxyphenol, 3-hydroxy-4,5-dimethyl-2(5H)-furanone, hexadecanol, octadecanal, 2.3-pentanedione, butanoic acid, β-iso-methyl ionone, β-ionone, were from Sigma-Aldrich (St. Louis MO). Stock standard solutions 103 or 104 µg mL−1 of each compound was prepared as described earlier [21 (link)].
3
Preparation of Analytical Standards
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Dichloromethane HiPerSolv (DCM) and sodium sulfate (Na 2 SO 4 ) were provided by VWR International GmbH (Darmstadt, Germany). DCM was freshly distilled prior to use. The analytical standards 1,8-cineole, linalool, cuminaldehyde, cinnamaldehyde, sotolone, 4-hydroxy-2,5-dimethyl-3(2H)-furanone, eugenol, vanillin and 𝛾-nonalactone were purchased from Sigma Aldrich (Steinheim, Germany). The isotopically labeled standards 2 H 3 -1,8-cineole, 2 H 4-5 -linalool, 2 H 8 -cuminaldehyde, 2 H 6cinnamaldehyde, 13 C 2 -sotolone, 13 C 2 -4-hydroxy-2,5-dimethyl-3(2H)-furanone, 2 H 3 -eugenol and 2 H 4 -𝛾-nonalactone were from aromaLAB GmbH (Martinsried, Germany) and 13 C 6 -vanillin from Sigma Aldrich.
4
Aroma Compound Analysis Protocol
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The following aroma compounds were purchased from Sigma-Aldrich (purity in parenthesis): acetaldehyde (≥99 %), acetic acid (≥99.5 %), 2,3-butanedione (97 %), butanoic acid (≥99 %), dimethyl sulfide (≥99 %), 5(or 2)-ethyl-4-hydroxy-2(or 5)-methyl-3(2H)-furanone (homofuraneol, 96 %), Z-4-heptenal (≥98 %), 3-hydroxy-4,5-dimethyl-2(5H)-furanone (sotolone, 10 % in propylene glycol), 4-hydroxy-2,5-dimethyl-3(2H)-furanone (furaneol, ≥98 %), methional (≥97 %), 2'methoxyacetophenone (99 %), 2-methoxy-4-methylphenol (≥98 %), 2-methoxyphenol (≥99 %), 2methoxy-4-vinylphenol (≥98%), 2-methylbutanal (≥95 %), 3-methylbutanal (≥97 %), 3methylbutanoic acid (99 %), 3-methyl-1-butanol (≥98%), methylpropanal (≥98 %), 2methylthiophene (98 %), 2,3-pentanedione (≥96 %), phenylacetaldehyde (10 % in ethanol), 2phenylacetic acid (≥99 %), 2-phenylethanol (≥99 %), vanillin (≥97 %), 4-vinylphenol (10 % in propylene glycol). All were food grade except 2'-methoxyacetophenone and 2-methylthiophene.
5
Development of DNA Aptamer Library
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Sodium chloride, magnesium chloride, potassium chloride, calcium chloride, 2-amino-2-(hydroxymethyl)-1,3-propanediol (Trizma base), hydrochloric acid, 2,5-dimethyl-4-hydroxy-3(2H)-furanone (furaneol), 5-Ethyl-3-hydroxy-4-methyl-2(5H)-furanone (maple furanone), 4,5-Dimethyl-3-hydroxy-2,5-dihydrofuran-2-one (sotolon), 5-Ethyl-4-hydroxy-2-methyl-3(2H)-furanone (homofuraneol), 2-Methoxyphenol (guaiacol), and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) were purchased from Sigma-Aldrich (Munich, Germany). Taq DNA polymerase, dNTPs solution, TAE and TBE electrophoresis buffers, DNA extraction kits, DNA cloning kit, Exonuclease I, and the Escherichia coli DH5α strain were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SYBR Gold dye was purchased from Invitrogen (Thermo Fisher Scientific). SYBR Green I dye was obtained from Lumiprobe Ltd., (Moscow Russia). Streptavidin-coated magnetic beads were supplied by New England Biolabs (Ipswich, MA, USA).
The single-stranded DNA library B1_bank was synthesized by DNA Synthesis Ltd., Moscow, Russia. All primers for DNA amplification were ordered from the DNA Synthesis Ltd., Moscow, Russia. The B1_cap was either purchased from DNA Synthesis Ltd., Moscow, Russia or from Microsynth AG (Balgach, Switzerland). Candidate aptamer sequences were synthesized by Evrogen Ltd. (Moscow, Russia).
The single-stranded DNA library B1_bank was synthesized by DNA Synthesis Ltd., Moscow, Russia. All primers for DNA amplification were ordered from the DNA Synthesis Ltd., Moscow, Russia. The B1_cap was either purchased from DNA Synthesis Ltd., Moscow, Russia or from Microsynth AG (Balgach, Switzerland). Candidate aptamer sequences were synthesized by Evrogen Ltd. (Moscow, Russia).
6
Comprehensive Soy Sauce Profiling
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A total of 32 different kinds of soy sauce, coded as XH1, XH2, XH3, XH4, XH5, XH6, XH7, XH8, YZ, HT1, HT2, HT3, HT4, JJ1, LJJ2, LJJ3, LJJ4, LJJ5, CB1, CB2, CB3, CB4, JJ1, JJ2, LCC1, LCC2, LCC3, BT1, BT2, HJ, WZ, and MG were used in this work. All samples were purchased from a local supermarket. All samples were stored in their original containers and sealed at room temperature (25 °C) prior to analyzsise.
Chromatographic grade methanol (99.9%) was purchased from Merck Chemical Company (Shanghai, China). 2-Methyl-3-heptanone (99.9%), and 5-ethyl-4-hydroxy-2-methyl-3(2H)furanone (99.9%) were purchased from Macklin Biochemical Company (Shanghai, China). 2,5-Dimethyl-4hydroxy-3(2H)-furanone (99.9%) was purchased from Sigma-Aldrich (Steinheim, Germany). Ethyl acetate (99.9%), 4-ethyl-2-methoxyphenol (99.9%), and 3-methylbutanal (99.9%) were purchased from J&K Scientific (Beijing, China). C6–C28 n-alkanes standard mixtures (≥97%) were purchased from Sigma Aldrich (Steinheim, Germany). Purified water was purchased from Hangzhou Wahaha Co., Ltd., Hangzhou, China.
Chromatographic grade methanol (99.9%) was purchased from Merck Chemical Company (Shanghai, China). 2-Methyl-3-heptanone (99.9%), and 5-ethyl-4-hydroxy-2-methyl-3(2H)furanone (99.9%) were purchased from Macklin Biochemical Company (Shanghai, China). 2,5-Dimethyl-4hydroxy-3(2H)-furanone (99.9%) was purchased from Sigma-Aldrich (Steinheim, Germany). Ethyl acetate (99.9%), 4-ethyl-2-methoxyphenol (99.9%), and 3-methylbutanal (99.9%) were purchased from J&K Scientific (Beijing, China). C6–C28 n-alkanes standard mixtures (≥97%) were purchased from Sigma Aldrich (Steinheim, Germany). Purified water was purchased from Hangzhou Wahaha Co., Ltd., Hangzhou, China.
7
Volatile Compound Standards for Food Analysis
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The volatile standards, i.e. phenylacetaldehyde (95%), 3-methylbutanoic acid (99%), 3-(methylthio)propanal (98%), 4-vinylguaiacol (≥98%), ethyl isobutyrate (99%), 3hydroxy-2-methyl-4-pyrone (maltol, 99%), 2,5-dimethyl-4-hydroxy-3(2H)-furanone (HDMF, ≥ 99%), 5-ethyl-4-hydroxy-2-methyl-3(2H)-furanone (HEMF, mixture of isomers, 96%) and standards of n-alkanes (C6-C33, 99%) were purchased from Sigma-Aldrich (Steinheim, Germany). 3-Methyl-1-butanol (99.8%), ethyl butyrate (≥99.5%), 1-octen-3-ol (98%), 2-methylbutanoic acid (98%), 4-ethyl-2-methoxyphenol (98%), 2phenylethanol (98%), dimethyl trisulfide (98%), 3-(methylthio)-1-propanol (99%) and 2,5-dimethylpyrazine (98%) were purchased from Aladdin (Shanghai, China). In the GC/O-AT and sensory evaluation sessions, nine food-grade volatile compounds, including phenylacetaldehyde (>99%), 3-hydroxy-2-methyl-4-pyrone (>96%), 2,5dimethyl-4-hydroxy-3(2H)-furanone (>98%), 3-(methylthio)propanal (>98%), 1octen-3-ol (>96%), 3-(methylthio)-1-propanol (>99%), dimethyl trisulfide (>95%), 2,5-dimethylpyrazine (>99%), and 5-ethyl-4-hydroxy-2-methyl-3(2H)-furanone (>98%), were purchased from Huabao Co. (China). Distilled water was obtained from Watsons Co. (Guangdong, China). Food-grade sodium chloride was purchased from Xiangheng Co. (Hunan, China).
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