Immunodeficient nsg nod ltsz scid il2rγnull mice

Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells, LLC) were infected with various amounts of each virus as indicated in the results section (2,000 to 20,000 GAUs) in vitro for 1.5 hours at 37°C, and a minimum of 10 million cells were injected intraperitoneally (i.p.) into 3–5 weeks-old age matched NSG mice [83 (link)]. Mice were kept for maximum time of 42 days after cord blood injection or were euthanized due to tumor symptoms prior to 42 days.

Immunodeficient NSG (NOD/LtSz-scid/IL2Rγ^null) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells) were infected with 5000 Green Raji Units of wild-type or mutant EBV strains in vitro for 1 ½ hours at 37°C, and a minimum of 10 million cells were injected intraperitoneally into 3 to 5-week-old NSG mice which were age-matched [51 (link),53 (link)]. Mice were kept for a maximum of 90 days after injection of cord blood, or were euthanized due to tumor symptoms prior to day 90. Studies were performed using three different donors.