Anti 4e bp1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-4E-BP1 is a laboratory reagent that specifically recognizes and binds to the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1). 4E-BP1 is a regulator of protein synthesis and plays a role in cell growth and proliferation. Anti-4E-BP1 can be used for the detection and quantification of 4E-BP1 in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti 4e bp1

Immunoblotting Analysis of Branched-Chain Protein Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins separated by 2-DE or SDS-PAGE were transferred to a PVDF membrane and then immunoblotted with anti-BCKDE1α (branched chain keto acid dehydrogenase E1 alpha) (#sc-271538, Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-OAT (ornithine aminotransferase) (#sc-374243, Santa Cruz Biotechnology, Inc.), anti-ODC (ornithine decarboxylase) (#sc-33539, Santa Cruz Biotechnology, Inc.), anti-S6K1 (ribosomal protein S6 kinase beta-1) (#sc-230, Santa Cruz Biotechnology, Inc.) and anti-4E-BP1 (eukaryotic translation initiation factor 4E-binding protein 1) (#sc-6936, Santa Cruz Biotechnology, Inc.) antibodies. After incubation with appropriate secondary antibodies, the proteins were visualized using enhanced chemiluminescence (ECL, GE Healthcare). Pixel intensities of the immunoreactive bands were quantified using the Image Master 2D Platinum 5.0 software (GE Healthcare) or Image Lab software (Bio-Rad). The phosphorylation of S6K1 and 4E-BP1 was determined as described previously [12 (link),13 (link),14 (link)].

Koike S., Kabuyama Y., Obeng K.A., Sugahara K., Sato Y, & Yoshizawa F. (2020). An Increase in Liver Polyamine Concentration Contributes to the Tryptophan-Induced Acute Stimulation of Rat Hepatic Protein Synthesis. Nutrients, 12(9), 2665.

+ Open protocol

+ Expand

Cellular Stress Induction and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

To induce cellular stress, sodium arsenite (arsenite, NaAsO₂; Sigma-Aldrich) and potassium tellurite (tellurite, K₂TeO₃ kindly gifted by Dr. José Manuel Pérez-Donoso, UNAB, Chile) were used. Cycloheximide and Puromycin were obtained from Sigma Aldrich. The antibodies used in this work included the following: anti-phospho-eIF2α (rabbit polyclonal) and anti-GAPDH (mouse monoclonal) from Abcam; anti-eIF2α (mouse monoclonal), anti-phospho-S6K1 (rabbit monoclonal), anti-phospho-S6 (rabbit monoclonal), anti-phospho-4EBP1 (rabbit monoclonal) from Cell Signaling Technology; anti-Puromycin (mouse monoclonal) from Millipore; anti-DCP1a (mouse monoclonal), anti-eIF3b (mouse monoclonal), anti-TIAR (goat polyclonal), anti-DDX3 (rabbit polyclonal), anti-phospho-mTOR (mouse monoclonal), anti-mTOR (mouse monoclonal), anti-S6K1 (mouse monoclonal), anti-S6 (mouse monoclonal), anti-4EBP1 (mouse monoclonal) from Santa Cruz Biotechnology and anti-SC35 (mouse monoclonal) kindly provided by Dr. Verónica Noches, PUC, Chile. Secondary antibodies used for immunofluorescence included the following: Alexa Fluor 594 donkey anti-mouse, Alexa Fluor 594 donkey anti-rabbit and Alexa Fluor 647 donkey anti-mouse or anti-goat from Life Technologies; and secondary antibodies for western blot (horseradish peroxidase-conjugated) anti-mouse and anti-rabbit were purchased from Jackson Immunoresearch.

Gaete-Argel A., Velásquez F., Márquez C.L., Rojas-Araya B., Bueno-Nieto C., Marín-Rojas J., Cuevas-Zúñiga M., Soto-Rifo R, & Valiente-Echeverría F. (2021). Tellurite Promotes Stress Granules and Nuclear SG-Like Assembly in Response to Oxidative Stress and DNA Damage. Frontiers in Cell and Developmental Biology, 9, 622057.

+ Open protocol

+ Expand

Molecular Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue culture reagents were obtained from GE Healthcare (Carlsbad, CA, USA).
MnCl₂, D-Aspartic, and the MTT reagent were purchased from
Sigma-Aldrich (St. Louis, MO, USA). L-Glutamate was obtained from Tocris
Biosciences (Ellisville, IL, USA). L-[³⁵S]-Methionine (Cat# NEG009A,
specific activity 1175 Ci/mmol) was purchased from PerkinElmer (Waltham MA,
USA). Bradford and sodium dodecyl sulfate-polyacrylamide gel electrophoresis
(SDS-PAGE) reagents were obtained from Bio-Rad (Hercules, CA, USA). Anti-pAkt
(Cat# sc-514032, RRID: AB_2861344), anti-Akt (Cat# sc-81434, RRID:AB_1118808),
anti-p4E-BP1 (Cat# sc-23768-R, RRID: AB_2095599), anti-4E-BP1 (Cat# sc-9977,
RRID:AB_626621) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA);
anti-pAMPK (Cat# 2535, RRID:AB_331250), anti-AMPK (Cat# 2532, RRID:AB_330331),
anti-ERK1/2 (Cat# 9102, RRID:AB_330744) were purchased from Cell Signaling
(Danvers, MA USA). Secondary antibodies were from Abcam. Horseradish
peroxidase–linked secondary antibodies and the enhanced chemiluminescence
reagent (ECL) were obtained from GE Healthcare (Carlsbad, CA, USA). All other
chemicals were from Sigma (St. Louis, MO, USA).

Soto-Verdugo J., Siva-Parra J., Hernández-Kelly L.C, & Ortega A. (2022). Acute Manganese Exposure Modifies the Translation Machinery via PI3K/Akt Signaling in Glial Cells. ASN NEURO, 14, 17590914221131452.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!