The equilibrium azithromycin solubility was determined. Briefly, an excess of azithromycin (i.e., > 5 mg/mL equivalent of azithromycin of each formulation) was added to 5 ml of dissolution media (phosphate buffer saline, PBS (pH 7.4), potassium chloride buffer (pH 2) and carbonate buffer (pH 11) maintained at 37 ± 1°C. Buffer media were used instead of water to reflect potential buffering effects of lung lining fluid. The resultant suspensions were constantly stirred at 500 rpm (VWR International, Arlington Heights, Illinois, USA) with a magnetic bar (12 mm, VWR, Arlington Heights, Illinois, USA). After 24 h the suspensions were filtered using a 0.45 μm nylon syringe filter (VWR, Arlington Heights, Illinois, USA) and the concentrations of azithromycin was determined by HPLC.
0.45 μm nylon syringe filter
Manufactured by Avantor
Sourced in United States
The 0.45 μm nylon syringe filter is a laboratory filtration device used to remove particulates and other contaminants from liquid samples. It features a 0.45 μm pore size nylon membrane that effectively traps particles while allowing the desired liquid to pass through.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 0.45 μm nylon syringe filter
1
Azithromycin Solubility Determination
2
Natural pH Leaching of Coal Ash
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Natural pH leaching procedure was adapted from LEAF Protocol Method 1316 (United States Environmental Protection Agency, 2012b ). Each coal ash material was mixed with Milli-Q water (2 g dry ash to 30 mL Milli-Q water) and tumbled end over end at 28 RPM for 24 h. The samples were then centrifuged at 4,000 RPM for 10 min and the supernatant was decanted and a portion was measured for pH. The remaining supernatant was filtered through a 0.45-μm nylon syringe filter (VWR) and diluted and acidified for ICP-MS analysis with 1–2% nitric acid to ensure that pH <2. The ash materials were extracted in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!