γ h2ax

Manufactured by Bioworld Technology

Sourced in China

The γ-H2AX is a laboratory equipment used to detect and quantify DNA double-strand breaks. It functions by measuring the phosphorylation of the histone protein H2AX, which is a cellular response to DNA damage.

Automatically generated - may contain errors

Lab products found in correlation

Stannous octoate sn oct 2, by Merck Group (2 mentions) Cd 31 polyclonal antibodies, by Bioworld Technology (2 mentions) Hif 1α, by Bioworld Technology (2 mentions) Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Hyclone, by Thermo Fisher Scientific (1 mentions) Bovine testicular hyaluronidase, by Meilun (1 mentions) Tyramine hydrochloride, by Meilun (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Mpeg mn 2000, by Merck Group (1 mentions) ε caprolactone, by Thermo Fisher Scientific (1 mentions) Ki 67, by Bioworld Technology (1 mentions)

4 protocols using γ h2ax

Targeted Folate-Conjugated Nanoparticles

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Ola with a purity level of 99.8% was purchased from Meilun Co., Ltd (Dalian, China). ε-Caprolactone (ε-CL), poly (ethylene glycol) (PEG, Mn=4000), and stannous octoate (Sn (Oct)₂) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), N,N’-Diisopropylcarbodiimide (DIC), MTT, and fluorescein isothiocyanate (FITC) were purchased from Sigma-Aldrich. Further, dimethyl sulfoxide (DMSO), folate, petroleum ether, anhydrous ethanol, acetonitrile, and methanol (HPLC grade) were purchased from KeLong Co., Ltd (Chengdu, China). Tetrahydrofuran (THF) was purchased from Bioway (New York, NY). γ-H2AX, Ki-67, and CD-31 polyclonal antibodies were purchased from Bioworld Technology Co., Ltd (Nanjing, China).
Hela and A549 cell lines were provided by Zhongke Quality Inspection Biotechnology Co., Ltd (Beijing, China). BALB/c mice (female, nu/nu, 4–5 weeks old) were purchased from Chongqing TengXin biotechnology Co., Ltd (Chongqing, China).

Li D., Hu C., Yang J., Liao Y., Chen Y., Fu S.Z, & Wu J.B. (2020). Enhanced Anti-Cancer Effect of Folate-Conjugated Olaparib Nanoparticles Combined with Radiotherapy in Cervical Carcinoma. International Journal of Nanomedicine, 15, 10045-10058.

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Sodium Hyaluronate-Tyramine Hydrogel Synthesis

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Sodium hyaluronate (HA) (>95%, Mw = 90 kDa), tyramine hydrochloride (Tyr·HCl), 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC·HCl), Hydrogen peroxide (H₂O₂, wt. 30%), N-hydroxysuccinimide (NHS), Horseradish peroxidase (HRP, 400–1,000 U/mg) and bovine testicular hyaluronidase were all purchased from MeiLun Co. Ltd. (Dalian, China). AL (dihydrochloride form; purity >99.9%) was obtained from Jiangsu Chia-tai Tianqing Pharmaceutical Co, Ltd (Nanjing, China). Crystal violet was purchased from Kelong Co. Ltd. (Chengdu, China). Polyclonal antibodies against Ki-67, VEGF-A, HIF-1α, and γ-H2AX were purchased from Bioworld Technology Co. Ltd. (Nanjing, China).
LLCs and human umbilical endothelial cells (HUVECs) were obtained from the experimental laboratory of Southwest Medical University (Luzhou, Sichuan, China) and cultured in a humidified 5% CO₂ atmosphere at 37°C with Dulbecco’s Modified Eagle’s medium (HyClone; Thermo Scientific, Waltham, MA, USA), 10% fetal bovine serum (HyClone, Thermo Scientific), 0.1 mg/ml streptomycin and 100 U/ml penicillin.

Gao Q., Jiang Y., Li X., Chen H., Tang S., Chen H., Shi X., Chen Y., Fu S, & Lin S. (2021). Intratumoral Injection of Anlotinib Hydrogel Combined With Radiotherapy Reduces Hypoxia in Lewis Lung Carcinoma Xenografts: Assessment by Micro Fluorine-18-fluoromisonidazole Positron Emission Tomography/Computed Tomography Hypoxia Imaging. Frontiers in Oncology, 11, 628895.

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Mouse Lung Cancer Xenograft Model

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C57BL/6 mice (female, aged 6-8 weeks) were obtained from the Chengdu Dashuo biotechnology Co. Ltd. (Chengdu, China). All animal experiments were approved by the Institutional Animal Care and Treatment Committee of Southwest Medical University (Luzhou, China). All mice were treated humanely. Lewis lung carcinoma cell lines (LLC) were obtained from the Experimental Research Center of the A liated Hospital of Southwest Medical University (Luzhou, China) or from the Cell Bank of the Chinese Academy of Sciences. Cell lines were grown in RPMI-1640 (1640, Gibco, USA) supplemented with 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin at 37 °C with 5% CO 2 . Bi dobacterium infantis (Bi) was purchased from the Guangdong culture collection center (Guangdong, China). Speci c monoclonal bi dobacterium antibodies (Ab) were prepared using AbMax Biotechnology Co., Ltd (Beijing, China). γ-H2AX, CD31, Ki-67, Glut-1, HIF-1α and TNF-α polyclonal antibodies were purchased from Bioworld technology Co. Ltd. (Nanjing, China).

Yang J., Wu Z., Chen Y., Hu C., Li D., Liao Y., Li Y., Wen Q., Chen Y., Fu S., & Wu J. (2020). A novel radio-sensitization method for lung cancer therapy: enhanced radiosensitization induced by antigens/antibodies reaction after targeting tumor hypoxia using Bifidobacterium.

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Polymer Synthesis and Cellular Assays

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Ola with 99.8% purity was purchased from Meilun Co., Ltd (Dalian, China). MPEG (Mn =2,000) was purchased from Sigma-Aldrich (St Louis, MO, USA), ε-caprolactone from Alfa Aesar (Reston, VA, USA), and stannous octoate (Sn(Oct)₂) purchased from Sigma-Aldrich. Dimethylsulfoxide (DMSO), anhydrous ethanol, and methanol (HPLC grade) were purchased from KeLong Co., Ltd (Chengdu, China). γ-H2AX, CC3, Ki-67, and CD-31 polyclonal antibodies were purchased from Bioworld Technology Co. Ltd. (Nanjing, China).

Wu M., Liu J., Hu C., Li D., Yang J., Wu Z., Yang L., Chen Y., Fu S, & Wu J. (2018). Olaparib nanoparticles potentiated radiosensitization effects on lung cancer. International Journal of Nanomedicine, 13, 8461-8472.

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