At indicated time points, cornea (both corneas per animal pooled together) or TG (both branches of TG per animal pooled together) samples were homogenized with a tissue miser, clarified by centrifugation (10,000g for 1.5 minutes), and then serially diluted onto a confluent lawn of Vero cells in RPMI1640 media (Invitrogen, Life Sciences, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) and antibiotic/antimycotic reagents. Plaques were visualized and enumerated with the aid of an inverted microscope (Invertoskop, Zeiss, Thornwood, NY, USA) 28 to 32 hours later and quantified as mean log PFU per cornea or log PFU per TG samples as previously described.19 (link),41 (link)
Invertoskop
Manufactured by Zeiss
Sourced in United States
The Invertoskop is a high-quality optical microscope designed for versatile laboratory applications. It features a sturdy construction and precise optics to deliver clear and detailed images. The Invertoskop is capable of both brightfield and darkfield observation modes, allowing for a wide range of sample examination.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using invertoskop
1
Viral Plaque Assay for Cornea and Trigeminal Ganglion
2
Microparticle Core Engulfment Efficiency
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