All the chemicals were used as received and purchased from Sigma‐Aldrich or Fluorochem unless otherwise noted. Birch wood, pine, reed, and walnut shells were picked up from local sources. The catalyst used for RCF/hydrogenolysis was 5 % Ru on carbon and aluminum oxide (Sigma). p‐Coumuric acid (pCCA), ferulic acid (FAA), xylan from birch, and Avicel®PH‐101 cellulose from cotton were also purchased from Sigma‐Aldrich. Solvents were supplied by Fisher Scientific. Some standard chemicals including 4‐(3‐hydroxypropyl)‐2,6‐dimethoxyphenol (S1), 2,6‐dimethoxy‐4‐propylphenol (S2), 4‐ethyl‐2,6‐dimethoxyphenol (S3), methyl 3‐(4‐hydroxy‐3‐methoxyphenyl)propanoate (FMA), methyl (E)‐3‐(4‐hydroxy‐3‐methoxyphenyl)acrylate (FAMe), methyl 3‐(4‐hydroxyphenyl)propanoate (PMA), hydroxycyclohexyl)propanoate (OHP), and methyl (E)‐3‐(4‐hydroxyphenyl)acrylate (pCAMe) were synthesized and isolated for this study, (S1.5, Figures S1–11). More detailed information on the synthetic and analytical procedures can be found in the Supporting Information.
Avicel ph 101 cellulose
Manufactured by Merck Group
Avicel® PH-101 is a microcrystalline cellulose (MCC) product manufactured by the Merck Group. It is a purified, partially depolymerized cellulose, used as a functional excipient in pharmaceutical and nutritional formulations.
Automatically generated - may contain errors
3 protocols using avicel ph 101 cellulose
1
Valorization of Lignocellulosic Biomass
2
Binding of CuGE to Insoluble Biopolymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To assess the effect of the CBM1 module on the binding of CuGE to insoluble biopolymers a pull-down assay was performed. Suspensions of 10 mg mL−1 biopolymer, including Avicel® PH-101 cellulose (Sigma-Aldrich), xylan from birchwood (Sigma-Aldrich), and LRP, were prepared in 50 mM sodium acetate buffer pH 5. Full length and truncated CuGE variants, WT and ∆WT, respectively, were added to a final concentration of 0.07 mg mL−1 in a total reaction volume of 550 µL. The samples were incubated for 1 h at 4 ◦C with rotation. After incubation the reaction tubes were centrifuged at 14,000 rpm for 10 min to separate pellet and supernatant. The pellet, containing protein bound to the insoluble biopolymer, was washed in water twice and then re-suspended in 550 µL water. To increase the protein concentration all samples were freeze dried and re-suspended in 100 µL prior to analysis. Samples were reduced with DTT and analyzed by SDS-PAGE using Mini-PROTEAN® TGX™ Precast Gels (Bio-RAD).
3
Preparation of Cellulose-Based Electrolyte
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microcrystalline Avicel® PH-101 cellulose was purchased from Sigma-Aldrich and was dried at 80 °C under vacuum for 12 h before use. N-Butyl-N-methylpyrrolidinium chloride (>99%) was also obtained from Sigma-Aldrich. Silver oxide (99%) and activated charcoal powder from the Wako brand were used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!