Anti dot1l antibody

Manufactured by Thermo Fisher Scientific

The Anti-DOT1L Antibody is a laboratory research tool used to detect and study the DOT1L protein, which is involved in the regulation of gene expression. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to analyze the presence and distribution of the DOT1L protein in biological samples.

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Lab products found in correlation

Ez magna rip kit, by Merck Group (2 mentions) Ab56919, by Abcam (1 mentions) Ab10485, by Abcam (1 mentions) Anti wdr5 antibody, by Abcam (1 mentions) Anti kat3b p300 antibody, by Abcam (1 mentions) Anti tet1 antibody, by Abcam (1 mentions)

2 protocols using anti dot1l antibody

RIP Assays of Epigenetic Regulators

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RIP assays were performed using the EZ-Magna RIP Kit (Millipore). Briefly, 1 × 10⁷ cells were lysed with RIP lysis buffer. Cell extracts were co-immunoprecipitated with Anti-TET1 antibody (Active motif, 61741, 2 µg per 10⁷ cell lysate), Anti-KAT3B/p300 antibody (Abcam, ab10485, 2 µg per 10⁷ cell lysate), Anti-WDR5 antibody (Abcam, ab56919, 2 µg per 10⁷ cell lysate), and Anti-DOT1L Antibody (Thermo Fisher, A300-953A, 2 µg per 10⁷ cell lysate). The recovered RNA was subjected to RT-qPCR analysis and U1 was used as a nonspecific control target.

Zhou T., Zhu X., Ye Z., Wang Y.F., Yao C., Xu N., Zhou M., Ma J., Qin Y., Shen Y., Tang Y., Yin Z., Xu H., Zhang Y., Zang X., Ding H., Yang W., Guo Y., Harley J.B., Namjou B., Kaufman K.M., Kottyan L.C., Weirauch M.T., Hou G, & Shen N. (2022). Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Nature Communications, 13, 1855.

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RNA-Protein Interaction Profiling

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RIP assays were performed using the EZ-Magna RIP Kit (Millipore). 1×10 7 cells were lysed with RIP lysis buffer. Cell extracts were coimmunoprecipitated with Anti-TET1 antibody (Abcam, ab191698), Anti-KAT3B/p300 antibody (Abcam, ab10485), Anti-WDR5 antibody (Abcam, ab56919), and Anti-DOT1L Antibody (Thermo, A300-953A).
The recovered RNA was subjected to RT-qPCR analysis and U1 was used as a nonspecific control target.

Zhou T., Zhu X., Ye Z., Wang Y., Yao C., Xu N., Zhou M., Ma J., Qin Y., Shen Y., Tang Y., Yin Z., Xu H., Zhang Y., Zang X., Ding H., Yang W., Guo Y., Harley J.B., Namjou B., Kaufman K.M., Kottyan L.C., Weirauch M.T., Hou G., & Shen N. (2021). CRISPRa screen on a genetic risk locus shared by multiple autoimmune diseases identifies a dysfunctional enhancer that affects IRF8 expression through cooperative lncRNA and DNA methylation machinery.

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