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Soluble anti cd3 monoclonal antibody mab

Manufactured by BioXCell
Sourced in United States

The Soluble anti-CD3 monoclonal antibody (mAb) is a laboratory reagent used for research purposes. It targets the CD3 receptor, which is part of the T-cell receptor complex. The core function of this product is to bind to and activate T cells in an in vitro setting.

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2 protocols using soluble anti cd3 monoclonal antibody mab

1

Splenocyte Cytokine Profiling Assay

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In a 24-well culture plate, splenocytes derived from C57BL/6N mice were cultivated using complete RPMI-1640 medium (2 × 106 cells/well). The splenocytes were then stimulated with heat-inactivated HDRsEf1 at various cell to bacteria ratios (10:1, 1:1, 1:10), LPS (100 ng/mL; Sigma-Aldrich, Saint Louis, MO, USA), or soluble anti-CD3 monoclonal antibody (mAb) (1 µg/mL, BioXcell, West Lebanon, NH, USA) for 3 days. After 3 days, the culture supernatant was collected for cytokine analysis.
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2

Splenocyte Cytokine Profiling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
In a 24-well culture plate, splenocytes derived from C57BL/6N mice were cultivated using complete RPMI-1640 medium (2 × 106 cells/well). The splenocytes were then stimulated with heat-inactivated HDRsEf1 at various cell to bacteria ratios (10:1, 1:1, 1:10), LPS (100 ng/mL; Sigma-Aldrich, Saint Louis, MO, USA), or soluble anti-CD3 monoclonal antibody (mAb) (1 µg/mL, BioXcell, West Lebanon, NH, USA) for 3 days. After 3 days, the culture supernatant was collected for cytokine analysis.
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