Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining kit (FITC-conjugated Annexin V) (eBioscience, USA) was used to label apoptosis cells. Briefly, 1 × 106 cells were resuspended in 0.5 ml staining binding buffer, and then, Annexin V-FITC (5 μl) and PI (1 μl) were added to the cells, respectively. Cells were stained for 15 min at room temperature and subjected to flow cytometry analysis.
Double staining kit fitc conjugated annexin 5
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FITC-conjugated Annexin V double staining kit is a laboratory tool used to detect and quantify apoptosis in cell samples. It contains Annexin V, a protein that binds to phosphatidylserine, a lipid that is exposed on the surface of cells undergoing apoptosis. The Annexin V is conjugated to the fluorescent dye FITC, allowing for visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.
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2 protocols using double staining kit fitc conjugated annexin 5
1
Apoptosis Detection by Annexin V-FITC/PI
2
Annexin V-FITC/PI Apoptosis Assay
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Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining kit (FITC-conjugated Annexin V) (eBioscience, USA) was used to label apoptosis cells. Briefly, 1 × 106 cells were resuspended in 0.5 ml staining binding buffer, and then, Annexin V-FITC (5 μ) and PI (1 μl) were added to the cells, respectively. Cells were stained for 15 min at room temperature and subjected to flow analysis. FlowJo software was used to analyze the results.
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