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Superoxide dismutase sod from bovine erythrocytes

Manufactured by Merck Group
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Sourced in United States, Germany

Superoxide dismutase (SOD) from bovine erythrocytes is a laboratory product offered by Merck Group. It is an enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide.

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Lab products found in correlation

Catalase, by Merck Group (2 mentions) Oxidized glutathione, by Thermo Fisher Scientific (1 mentions) Milli q advantage a10, by Merck Group (1 mentions) Celltiter 96 aqueous mts reagent powder, by Promega (1 mentions) Reduced glutathione, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) 5 5 dithiobis 2 nitrobenzoic acid dtnb, by Merck Group (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Z vad fmk, by Merck Group (1 mentions) Q vd oph, by R&D Systems (1 mentions) Propidium iodide, by Merck Group (1 mentions) Annexin 5 fluos, by Roche (1 mentions) Aβ25 35 fragment, by Merck Group (1 mentions) Diethylenetriaminepentaacetic acid dtpa, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Thioflavin t, by Merck Group (1 mentions) Potassium cyanide, by Merck Group (1 mentions) Xanthine oxidase from bovine milk, by Merck Group (1 mentions) Trichloroacetic acid, by Kemika (1 mentions) Butylated hydroxy toluene, by Thermo Fisher Scientific (1 mentions)

4 protocols using superoxide dismutase sod from bovine erythrocytes

1

Antioxidant and Cytotoxicity Evaluation

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Reduced glutathione, oxidized glutathione (purity 98%) and (–)-(1R,2S,5R)-menthol (purity 99.5%) were purchased from Acros Organics. 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB), tetraphosphorus decasulfide (purity 99%), Triton X100 and superoxide dismutase (SOD) from bovine erythrocytes were purchased from Sigma-Aldrich. Single-layer graphene oxide (purity 99%) was produced by Cheap Tubes Inc. CellTiter 96® Aqueous MTS Reagent Powder was purchased from Promega. Milli-Q grade water (Milli-Q® Advantage A10, Merck Millipore) was used to prepare buffers and solutions.
Ishkaeva R.A., Nizamov I.S., Blokhin D.S., Urakova E.A., Klochkov V.V., Nizamov I.D., Gareev B.I., Salakhieva D.V, & Abdullin T.I. (2021). Dithiophosphate-Induced Redox Conversions of Reduced and Oxidized Glutathione. Molecules, 26(10), 2973.
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2

Quantitative Analysis of Cellular Oxidative Stress

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Superoxide dismutase (SOD) from bovine erythrocytes, catalase, H2O2, ovalbumin, Z-VAD-fmk, propidium iodide (PI) and 4,5-diaminofluoresceína-diacetato (DAF-2DA) were purchase from Sigma (St. Louis, MO, USA). Apo DETECT ANNEXIN-V-FITC KIT 2,7-diclorodihidrofluoresceína-diacetato (DCF-DA) and dihidrorodamine-123 (DHR-123) were purchase from Invitrogen (Life Technologies, São Paulo, Brazil). Antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Q-VD-OPh (R&D Systems, Minneapolis, MN, USA), annexin-V-FLUOS (Roche, Mannheim, Germany).
Reis A.C., Alessandri A.L., Athayde R.M., Perez D.A., Vago J.P., Ávila T.V., Ferreira T.P., de Arantes A.C., de Sá Coutinho D., Rachid M.A., Sousa L.P., Martins M.A., Menezes G.B., Rossi A.G., Teixeira M.M, & Pinho V. (2015). Induction of eosinophil apoptosis by hydrogen peroxide promotes the resolution of allergic inflammation. Cell Death & Disease, 6(2), e1632-.
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3

Amyloid-beta Oxidative Damage Assay

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Aβ25-35 fragment (NH2- and COOH-terminal free peptide), superoxide dismutase (SOD) from bovine erythrocytes (EC 1.15.1.1), catalase, diethylenetriaminepentaacetic acid (DTPA), and Thioflavin-T were obtained from Sigma (St. Louis, MO, USA). l-methionine and hydrogen peroxide (30%) were purchased from Fluka (Buchs, Switzerland). All other chemicals were of the highest purity commercially available. H2O2 concentration was verified using UV absorption at 240 nm (ε = 43.6 M−1cm−1) [60 (link)]. All solutions and buffers were prepared with distilled water purified in a Millipore (Burlington, MA, USA) Milli-Q system and contained DTPA to avoid metal-dependent oxidative reactions.
Francioso A., Baseggio Conrado A., Blarzino C., Foppoli C., Montanari E., Dinarelli S., Giorgi A., Mosca L, & Fontana M. (2020). One- and Two-Electron Oxidations of β-Amyloid25-35 by Carbonate Radical Anion (CO3•−) and Peroxymonocarbonate (HCO4−): Role of Sulfur in Radical Reactions and Peptide Aggregation. Molecules, 25(4), 961.
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4

Glutathione Reductase Assay Protocol

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Glutathione reductase (GR) from baker's yeast (S. cerevisiae) (100-300 U mg -1 protein), superoxide dismutase (SOD) from bovine erythrocytes (3000 U mg -1 protein), xanthine oxidase from bovine milk (0.4-1.0 U mg -1 protein), ethylenediaminetetraacetic acid tetrasodium salt, nitrotetrazolium blue chloride (NBT), xanthine, and potassium cyanide were purchased from Sigma Aldrich (Germany). A stabilised 3 % solution of hydrogen peroxide was purchased from Fluka (Germany), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt ( N A D P H ) f r o m S e r v a ( G e r m a n y ) , a n d ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) from Pharmacia Biotech (Sweden). Acetonitrile and dimethyl sulfoxide (DMSO) were purchased from J.T. Baker (Italy), yeast extract (YES) from Biolife (Italy), while aflatoxin standard mix (AFB1, AFG1, AFB2, and AFG2) from Biopure (Austria). Sodium azide and hydrochloric acid were purchased from Merck (Germany), trichloroacetic acid from Kemika (Croatia), ethanol absolute from Panreac (Spain), and butylated hydroxytoluene and 2-thiobarbituric acid were purchased from Acros Organics (USA). All other chemicals were of p.a. quality and purchased from commercial suppliers.
Kovač T., Kovač M., Strelec I., Nevistić A, & Molnar M. (2017). Antifungal and antiaflatoxigenic activities of coumarinyl thiosemicarbazides against Aspergillus flavus NRRL 3251. Arhiv za higijenu rada i toksikologiju, 68(1).
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