Liposofast liposome factory

Manufactured by Merck Group

The LiposoFast Liposome Factory is a laboratory equipment designed for the production of liposomes. Liposomes are spherical vesicles composed of a lipid bilayer, which can be used for various applications in research and development. The LiposoFast Liposome Factory provides a controlled and efficient method for the formation of liposomes.

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Lab products found in correlation

Cholesterol, by Merck Group (1 mentions) Dotap, by Merck Group (1 mentions) Liposome kit, by Merck Group (1 mentions)

Spelling variants of this product

LiposoFast Factory (2 citations) LiposoFast (1 citations)

2 protocols using liposofast liposome factory

Liposome Preparation with DOTAP, DOPE, and Cholesterol

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DDC642 liposomes were generated as described by Desmet et al. [21 (link)]. Briefly, DOTAP (Sigma, #D6182-50mg, St.Louis, CA, USA), DOPE (Sigma, #42490-2.5 mL) and cholesterol (Sigma, #C8667-500 mg) were combined in a 6:4:2 ratio, incubated at 37 °C and resuspended in an EtOH/HEPES buffer. After incubation for 24 h at room temperature, the lipid solution was extruded 30 times through a 100-nm pore-sized polycarbonate membrane (Sigma, #Z373419-50EA) using a LiposoFast Liposome Factory (Sigma, #Z373400).

Ablinger M., Lettner T., Friedl N., Potocki H., Palmetzhofer T., Koller U., Illmer J., Liemberger B., Hainzl S., Klausegger A., Reisenberger M., Lambert J., Van Gele M., Desmet E., Van Maelsaeke E., Wimmer M., Zauner R., Bauer J.W, & Wally V. (2021). Personalized Development of Antisense Oligonucleotides for Exon Skipping Restores Type XVII Collagen Expression in Junctional Epidermolysis Bullosa. International Journal of Molecular Sciences, 22(7), 3326.

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Preparation of Uniform Liposomes

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A liposome kit purchased from Sigma-Aldrich (Catalog Number L4395, St. Louis, MO) was used. The user manual was followed to prepare multilamellar liposomes. Briefly, the lipid mixture was dissolved in CHCl₃/CH₃OH. The solution was dried under a stream of nitrogen. The vial containing the lipid film was placed under vacuum for 6 hrs to remove the traces of organic solvent. The dry lipid film was hydrated by adding PBS and vigorously shaking with a vortex mixer for >1 min. The MLV suspension were frozen and thawed for 6 cycles. A single freeze-thaw cycle consisted of freezing for 5 min at liquid nitrogen temperature (−196°C) and thawing for 5 min in a water bath at 65°C. Extrusion of the liposome suspension obtained was carried out with a filtration device (LiposoFast Liposome Factory, Sigma-Aldrich Chemicals) through a porous polycarbonate membrane with a pore size of 100 nm to obtain liposomes of uniform size. Size distribution of the liposomes prepared was measured by dynamic light scattering (DLS). Results are shown in Figure S1.

Xu R., Fitts A., Li X., Fernandes J., Pochampally R., Mao J, & Liu Y.M. (2016). Quantification of small extracellular vesicles by size exclusion chromatography with fluorescence detection. Analytical chemistry, 88(21), 10390-10394.

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