Ham s f 12k nutrient mixture

Manufactured by Corning

Sourced in United States

Ham's F-12K Nutrient Mixture is a cell culture medium formulation designed to support the growth and maintenance of various cell types, including mammalian cells. It provides the necessary nutrients, vitamins, and supplements required for cell proliferation and survival in vitro.

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Lab products found in correlation

Complete growth medium, by Celprogen (3 mentions) Sh sy5y cell, by American Type Culture Collection (3 mentions) Human endothelial progenitor cells, by Celprogen (3 mentions) Eagle s minimum essential medium, by Corning (3 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Bewo cells, by American Type Culture Collection (1 mentions) Trypsin edta solution, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) T 25 cell culture flask, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Atcc htb 96, by American Type Culture Collection (1 mentions) Atcc crl 1573, by American Type Culture Collection (1 mentions) Atcc ccl 1, by American Type Culture Collection (1 mentions) Atcc ccl 61, by American Type Culture Collection (1 mentions)

5 protocols using ham s f 12k nutrient mixture

Culturing Human Neuroblastoma and Endothelial Progenitor Cells

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham’s F-12 K Nutrient Mixture and Eagle’s Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO₂ at 37 °C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12–16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen, Torrance, CA) under standard cell culture conditions (5% CO₂, 37 °C) according to the manufacturer’s protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8–11 passages in the present study.

Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H, & Bihl J. (2020). miR-137 boosts the neuroprotective effect of endothelial progenitor cell-derived exosomes in oxyhemoglobin-treated SH-SY5Y cells partially via COX2/PGE2 pathway. Stem Cell Research & Therapy, 11, 330.

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Culturing Human Cell Lines for Research

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Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.

Li Y., Wang J., Chen S., Wu P., Xu S., Wang C., Shi H., & Chen J. (2020). MiR-137 Boosts the Neuroprotective Effect of Endothelial Progenitor Cell Derived-Exosomes in Oxyhemoglobin Treated SH-SY5Y Cells Partially via COX2/PGE2 Pathway.

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In Vitro Cultivation of Neuroblastoma and Endothelial Progenitor Cells

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Cell culture Human neuroblastoma cell line SH-SY5Y cells (ATCC, MD, USA) were cultured in complete medium containing Ham's F-12K Nutrient Mixture and Eagle's Minimum Essential Medium (Corning Cellgro, VA, USA) with 10% fetal bovine serum (HyClone, PA, USA), 100 IU/ml penicillin, and 100 mg/ml streptomycin in an atmosphere of 5% CO 2 at 37°C. The medium was changed every other day, the cells were passaged when the density reached around 75%. The 12 -16 passages of the cells were used in the present study.
Human endothelial progenitor cells (Celprogen, Torrance, CA) were cultured in complete growth medium (Celprogen; Torrance, CA) under standard cell culture conditions (5% CO 2 , 37°C) according to the manufacturer's protocol. The medium was changed every other day, and the cells were passaged when the density reached around 80%. The cells underwent 8-11 passages in the present study.

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BeWo Cell Culture and P. falciparum Enrichment

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BeWo cells (ATCC) were cultured in Ham’s F-12K nutrient mixture (Corning Cat. No. 10–025-CV) supplemented with 10% fetal bovine serum (ATCC Cat. No. 30–2020) and 40 μg/mL gentamicin (GIBCO Ref. No. 15750–060), at 37 °C in the presence of 5% CO₂. Medium was changed every 48 h until 80% confluence was reached. Cells were then trypsinized using Trypsin-EDTA solution (Sigma Cat. No. 59417C) containing 0.05% trypsin for 5 min at 37 °C, centrifuged at 400g for 5 min, and resuspended to desired concentration in the culture medium for further experiments. The CS2 strain of P. falciparum that expresses VAR2CSA and binds to CSA was grown and enriched following protocols published in previous research [30 (link)].

Liu J., Chesnokova O., Oleinikov I., Qiang Y., Oleinikov A.V, & Du E. (2020). Optimization of in vitro trophoblast assay for real-time impedimetric sensing of trophoblast-erythrocyte interactions in Plasmodium falciparum malaria. Analytical and bioanalytical chemistry, 412(16), 3915-3923.

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Cell Culture Protocols for Various Cell Lines

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Human cervix carcinoma Hep-2c cells (Merck, Darmstadt, Germany) were cultured in the RPMI 1640 medium (Corning Inc., Corning, NY, USA). Chinese hamster ovary CHO-K1 cells (ATCC^® CCL-61™; American Type Culture Collection, Manassas, VA, USA) were cultured in Ham’s F-12K Nutrient Mixture (Corning Inc., Corning, NY, USA). Human bone osteosarcoma U 2 OS cells (ATCC^® HTB 96™; American Type Culture Collection, Manassas, VA, USA), human embryonic kidney 293 [HEK-293] cells (ATCC^® CRL1573™; American Type Culture Collection, Manassas, VA, USA; referred to as cell line HEK-293), and mouse subcutaneous connective tissue NCTC clone 929 [L cell, L-929, derivative of Strain L] cells (ATCC^® CCL-1™; American Type Culture Collection, Manassas, VA, USA; referred to as cell line L-929) were cultured in the Dulbecco’s Modified Eagle Medium (DMEM; Corning Inc., Corning, NY, USA). The growth medium was supplemented with a 10% fetal bovine serum (FBS; Fisher Scientific, Waltham, MA, USA) for all cell lines. Cells were grown in T25 cell culture flasks (Fisher Scientific, Waltham, MA, USA) as a monolayer culture and routinely passaged after 90–100% confluency was reached. Cell cultures were maintained at 37 °C under a 5% CO₂, 95% air atmosphere.

Kavaliauskaitė J., Kazlauskaitė A., Lazutka J.R., Mozolevskis G, & Stirkė A. (2021). Pulsed Electric Fields Alter Expression of NF-κB Promoter-Controlled Gene. International Journal of Molecular Sciences, 23(1), 451.

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