Ros reagent kit

HT22 cells were obtained from the Xuzhou Medical University. Curcumin, Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), dimethyl sulfoxide (DMSO), and methylthiazolyldiphenyl-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lactic dehydrogenase (LDH) reagent kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). SOD2 activity assay kit was obtained from Trevigen (Gaithersburg, USA). The DAPI staining solution and ROS reagent kit were purchased from Beyotime Technology (Nantong, China). MitoSOX staining kits were purchased from Invitrogen Molecular Probes (San Diego, CA). The primary antibodies of anti-SOD2, anticleaved caspase-3, and anti-β-actin were purchased from Abcam (Cambridge, UK).

The PC12 cell-line was a gift from the Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China). Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium bromide (MTT), and nerve growth factor (NGF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The primary anti-SOD2 and anti-cleaved caspase-3 antibodies were purchased from Santa Cruz (USA). MitoTracker and MitoSOX staining kits were purchased from Invitrogen Molecular Probes (San Diego, CA). The 4′,6-diamidino-2-phenylindole (DAPI) staining solution and ROS reagent kit were purchased from Beyotime Technology (Nantong, China). The SOD activity assay kit was purchased from Trevigen (Gaithersburg, USA). The LDH, GSH, and CAT reagent kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).