A commercially available EdU labeling kit (C10314-3, RiboBio, China) was used according to the manufacturer's instructions, to detect hippocampal neuronal proliferation. EdU was administered to mice via intraperitoneal injection (5 mg/kg, dissolved at 1 mg/ml in phosphate-buffered solution), 24 h before their execution. After EdU labeling, Hoechst 33342 was used to label cell nuclei. All the images were collected on an Ultraview microscope (Olympus BX51TF, Japan), using standard excitation and emission filters for Hoechst 33342 and FITC (Alexa Fluor-488). For quantification, ×200 magnification fields were collected for each region of interest in the hippocampus, and for each coronal brain section. EdU positive cells were counted manually, viewing each image in Adobe Photoshop CS5.
C10314 3
Manufactured by RiboBio
Sourced in China
C10314-3 is a lab equipment product manufactured by RiboBio. It is a high-performance device designed for conducting scientific experiments and research. The core function of this product is to provide precise and reliable results for users. However, due to the need to maintain an unbiased and factual approach, a more detailed description cannot be provided without the risk of extrapolation or interpretation.
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2 protocols using c10314 3
1
Quantifying Hippocampal Neurogenesis with EdU
2
In Vivo EdU Proliferation Assay
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Mice were injected intraperitoneally with 50 mg/kg of EdU (C10314-3; Riobio, Guangzhou, China) 1 hr before killed. After washed with PBS for 15 min., frozen sections were fixed in paraformaldehyde for 30 min. After fixed, frozen sections were washed with PBS for three times, and then incubated with Cell-Light™ EdU Apollo®488 In Vivo Imaging Kit (C10314-3; Riobio). After incubation with EdU kit, sections were washed with PBS for three times, then incubated with DAPI (4′,6-diamidino-2-phenylindole, 1:500 dilution; Life Technology, Carlsbad, CA, USA). The images were taken under an amplification of 400× with confocal laser scanning microscope (LSM 710; Carl Zeiss MicroImaging GmbH, Jena, Germany). The results were expressed as EdU-positive cell number per mm2.
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