Pseudomonas aeruginosa atcc27853

Manufactured by Iranian Biological Resource Center

Pseudomonas aeruginosa ATCC27853 is a strain of the bacterium Pseudomonas aeruginosa. It is a Gram-negative, aerobic, rod-shaped bacterium that is widely used in research and laboratory settings. This strain is available from the American Type Culture Collection (ATCC) and is a common reference strain for various applications.

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Lab products found in correlation

Ferrous chloride tetrahydrate, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Silver nitrate, by Merck Group (1 mentions) Ammonia solution, by Merck Group (1 mentions)

2 protocols using pseudomonas aeruginosa atcc27853

Antimicrobial Activity Screening Protocol

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A starting bacterial suspension with a concentration of 0.5 McFarland was prepared for the target bacteria, including Staphylococcus aureus ATCC33591, Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 (Iranian Biological Resource Center, Iran). The bacteria were streaked on Mueller‐Hinton agar culture medium, and wells were created on the culture plates. Mueller‐Hinton medium (100 μL) was poured into each well, and then 0.005 g of the test sample were added. The plates were then placed in an incubator at 37°C for 24 h, and finally, after the incubation period, the formation of growth inhibition zones on the plates was examined.
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Haghparast‐Kenarsari T., Abdouss M., Heidari Keshel S., Heshmatipour Z., Rahimi A, & Biazar E. (2023). Improving properties of platelet‐rich fibrin scaffold with tannic acid for wound healing. International Wound Journal, 21(4), e14571.

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Magnetic Nanoparticle Synthesis and Characterization

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PVA, Mw: 85000–124000 g/mol, PCL, Mw: ~ 80000 g/mol and 3-(4,5-dimethylthiazol-2- yl)2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (USA). GA, dimethylformamide (DMF, 99.80%), chloroform (99.00–99.40%), silver nitrate (99.80%), ferrous chloride tetrahydrate (FeCl₂.4H₂O), ferric chloride heptahydrate (FeCl₃.7H₂O), oleic acid, and ammonia solution (32%) were supplied from Merck (Germany). All chemicals and solvents were of analytical grade and used without any further purification. Staphylococcus aureus (IBRC-M 10917) and Pseudomonas aeruginosa (ATCC 27853) were obtained from the Iranian Biological Resource Center (IBRC) and the American Type Culture Collection (ATCC), respectively. Mouse embryonic fibroblast (MEF) cells (RSCB0182) were obtained from the Royan Institute (Iran) for Stem Cell Biology and Technology.

Eghbalifam N., Shojaosadati S.A, & Hashemi-Najafabadi S. (2023). Role of bioactive magnetic nanoparticles in the prevention of wound pathogenic biofilm formation using smart nanocomposites. Journal of Nanobiotechnology, 21, 161.

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