Whole-cell extracts were obtained from cells washed with PBS, collected with Laemmli buffer (4% [vol/vol] SDS, 20% [vol/vol] glycerol, and 120 mM Tris-HCl, pH 6.8), and then sonicated with a Diagenode Bioruptor for 10 min followed by 5 min of boiling at 95°C before loading. Samples were resolved by SDS-PAGE and analyzed by standard WB protocols. Signals of the Western blots were detected by standard chemiluminescence (GE Healthcare) and analyzed using a ChemiDoc XRS+ system (Bio-Rad Laboratories). Primary antibodies used were rabbit anti-ZMYND8 (A302-089; Bethyl Laboratories, Inc.), rabbit anti-CHD4 (39289; Active Motif), rabbit anti-HDAC1 (ab19845; Abcam), rabbit anti-HDAC2 (ab7029; Abcam), rabbit anti-MTA2 (A300-395; Bethyl Laboratories, Inc.), mouse anti-KDM5A (ab78322; Abcam), rabbit anti-KDM5B (A301-813; Bethyl Laboratories, Inc.), rabbit anti-KDM5C (A301-034; Bethyl Laboratories, Inc.), rabbit anti-H3 (ab1791; Abcam), rabbit anti-H3K4me3 (9751; Cell Signaling Technology), rabbit anti–β-tubulin (ab6046; Abcam), rabbit anti-GFP (A11122 ; Invitrogen), mouse anti-Flag (F1804; Sigma-Aldrich), rabbit anti-ZNF592 (A301-530; Bethyl Laboratories, Inc.), rabbit anti-ZNF687 (A303-278; Bethyl Laboratories, Inc.), rabbit anti-RBMX (14794; Cell Signaling Technology), rabbit anti-DDB1 (A300-462; Bethyl Laboratories, Inc.), and mouse anti-RAD51 (ab88572; Abcam).
A301 813
Manufactured by Fortis Life Sciences
The A301-813 is a laboratory instrument designed for the quantitative analysis of various samples. It features high-precision measurements and reliable performance.
Automatically generated - may contain errors
Lab products found in correlation
Ab78322, by Abcam (2 mentions)
Ab6046, by Abcam (2 mentions)
A302 089, by Fortis Life Sciences (2 mentions)
A301 034, by Fortis Life Sciences (2 mentions)
Ab88572, by Abcam (1 mentions)
Ab19845, by Abcam (1 mentions)
Ab7029, by Abcam (1 mentions)
Ab1791, by Abcam (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Ab9084, by Abcam (1 mentions)
Ab37264, by Abcam (1 mentions)
A11122, by Thermo Fisher Scientific (1 mentions)
Par 4335 mc 100, by Bio-Techne (1 mentions)
Mabe1016, by Merck Group (1 mentions)
F1804, by Merck Group (1 mentions)
2 protocols using a301 813
1
Western Blot Analysis of Chromatin Regulators
2
Antibody Validation for KDM5 Proteins
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The following antibodies were used in this study: mouse anti-KDM5A (ab78322; Abcam), rabbit anti-KDM5B (A301-813; Bethyl Laboratories, Inc.), rabbit anti-KDM5C (A301-034; Bethyl Laboratories, Inc.), rabbit anti–β-tubulin (ab6046; Abcam), mouse anti-γH2AX (05-636, clone JBW301; Sigma-Aldrich), rabbit anti-GFP (A11122; Invitrogen), rabbit anti-PARP1 (9542; Cell Signaling Technology), mouse anti-PAR (4335-MC-100; Trevigen), rabbit anti–pan-ADP-ribose (MABE1016; Sigma-Aldrich), rabbit anti-MBP (Ab9084; Abcam), mouse anti-Flag (F1804; Sigma-Aldrich), rabbit anti-macroH2A1 (Ab37264; Abcam), rabbit anti-macroH2A1.1 (12455S [D5F6N]; Cell Signaling Technology), mouse anti-macroH2A1.2 (MABE61 [14G7]; Sigma-Aldrich), rabbit IgG (NI01; Calbiochem), mouse IgG (NI03; Calbiochem), and rabbit anti-ZMYND8 (A302-089; Bethyl Laboratories, Inc.).
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