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Sp5 flim confocal microscope

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The Leica SP5 FLIM confocal microscope is a high-performance imaging system designed for fluorescence lifetime imaging microscopy (FLIM). It provides accurate and reliable measurements of fluorescence lifetime, which can be used to study various biological processes and molecular interactions within samples. The SP5 FLIM combines the advanced confocal imaging capabilities of the Leica SP5 platform with the specialized FLIM functionality, allowing users to capture detailed, time-resolved fluorescence data.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Ab14730, by Abcam (1 mentions)

Spelling variants of this product

SP5 confocal microscope (1772 citations) Confocal microscope (1146 citations) SP5 microscope (176 citations) Confocal SP5 (6 citations) SP5 FLIM inverted confocal microscope (3 citations) TCS SP5 MP SMD FLIM confocal laser scanning microscope (2 citations)

2 protocols using sp5 flim confocal microscope

1

Visualizing Mitochondrial ATP Synthase

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Cells were grown on glass coverslips, fixed in 4% formaldehyde for 20 minutes, permeabilized with 0.1% Triton X-100 in Dulbecco’s PBS and blocked with 5% bovine serum albumin for 1 hour. Cells were incubated with mouse anti-ATPB (3D5) (1:1000; ab14730; abcam USA) overnight at 4°C, and then with mouse-Cy2-conjugated secondary antibodies (1:100; Jackson ImmunoResearch, PA, USA) for 1 hour at room temperature. Nuclei were counterstained by 4’,6-diamidino-2-phenylindole (DAPI, 1:10000; Thermo Fisher Scientific). Stained objects were imaged with a Leica SP5 FLIM confocal microscope.
Chandramouleeswaran P.M., Guha M., Shimonosono M., Whelan K.A., Maekawa H., Sachdeva U.M., Ruthel G., Mukherjee S., Engel N., Gonzalez M.V., Garifallou J., Ohashi S., Klein-Szanto A.J., Mesaros C.A., Blair I.A., Pellegrino da Silva R., Hakonarson H., Noguchi E., Baur J.A, & Nakagawa H. (2020). Autophagy mitigates ethanol-induced mitochondrial dysfunction and oxidative stress in esophageal keratinocytes. PLoS ONE, 15(9), e0239625.
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2

Histological Analysis of Mouse Tumors and Lungs

Cited 1 time
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For histological analysis, mouse tumor and mouse lung specimens were processed as previously described81 (link),82 (link). Antibodies information and dilutions are listed in Supplementary Table 2. DAPI was used to stain nuclei. IF Images were taken using a Leica SP5 FLIM Confocal microscope.
Kumar S., Nandi A., Singh S., Regulapati R., Li N., Tobias J.W., Siebel C.W., Blanco M.A., Klein-Szanto A.J., Lengner C., Welm A.L., Kang Y, & Chakrabarti R. (2021). Dll1+ quiescent tumor stem cells drive chemoresistance in breast cancer through NF-κB survival pathway. Nature Communications, 12, 432.
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