Shrna clones for ddr2

Manufactured by Thermo Fisher Scientific

ShRNA clones for DDR2 are laboratory reagents designed to facilitate the knockdown of the DDR2 gene. They provide a tool for researchers to study the biological function of the DDR2 protein.

Automatically generated - may contain errors

Lab products found in correlation

Snail1, by Thermo Fisher Scientific (2 mentions) Plenti6 vector, by Addgene (2 mentions) Trans lentiviral packaging system, by Addgene (2 mentions) Pentr vector, by Addgene (2 mentions) Lentivirus expression system, by Thermo Fisher Scientific (2 mentions) Blasticidin, by InvivoGen (2 mentions) Puromycin, by InvivoGen (2 mentions) Twist1, by Thermo Fisher Scientific (1 mentions)

2 protocols using shrna clones for ddr2

Overexpression and Knockdown of EMT Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Full-length FOXQ1, SNAIL1, TWIST1, ZEB2, and DDR2 plasmids were purchased from Open Biosystems. These genes were subcloned into the pENTR vector (RRID:Addgene_149548) and transferred into a pLenti6 vector (BRID: Addgene_21691) via homologous recombination. The lentivirus for the full-length gene was then generated using the lentivirus-expression system (Invitrogen). In addition, a set of short hairpin RNA (shRNA) clones for DDR2, FOXQ1, or SNAIL1 was purchased from Open Biosystems. The information for the effective shRNA is available in Supplementary Primers and shRNA information. The lentivirus for the shRNA was then generated using the Trans-Lentiviral packaging system (Addgene). The generated lentivirus was then used to infect the targeted model cells. Stable cells were generated after being selected with Blasticidin (10 μg /mL) for the overexpression model or puromycin (12 μg/mL; Invivogen) for the knockdown model.

Mitchell A.V., Wu J., Meng F., Dong L., Block C.J., Song W.M., Zhang B., Li J, & Wu G. (2022). DDR2 Coordinates EMT and Metabolic Reprogramming as a Shared Effector of FOXQ1 and SNAI1. Cancer Research Communications, 2(11), 1388-1403.

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Overexpression and Knockdown of EMT Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Full-length FOXQ1, SNAIL1, TWIST1, ZEB2, and DDR2 plasmids were
purchased from Open Biosystems (Huntsville, AL). These genes were sub-cloned
into the pENTR vector (RRID:Addgene_149548) and transferred into a pLenti6
vector (BRID: Addgene_21691) via homologous recombination. The lentivirus for
the full-length gene was then generated using the lentivirus-expression system
(Invitrogen, Carlsbad, CA). In addition, a set of shRNA clones for DDR2, FOXQ1,
or SNAIL1 was purchased from Open Biosystems. The information for the effective
shRNA is available in Supplementary Primers and shRNA information. The lentivirus for the
shRNA was then generated using the Trans-Lentiviral packaging system (Addgene,
Watertown, MA). The generated lentivirus was then used to infect the targeted
model cells. Stable cells were generated after being selected with Blasticidin
(10 μg /ml) for the overexpression model or puromycin (12 μg /ml)
(Invivogen, San Diego, CA) for the knockdown model.

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