1 oleoyl 2 hydroxy sn glycero 3 phosphocholine

Grade MS methanol (MeOH) and 2-propanol, ammonium formate, formic acid, acetic acid, methyl tert-buthyl ether (MTBE) and standards for calibration curves (1,3-dilinoleoyl-rac-glycerol [DG 36:4], 1-palmitoyl-2-oleoyl-3-linoleoyl-rac-glycerol [TG 52:3], 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine [LPC 18:1], 1,2-dioleoyl-sn-glycero-3-phosphocholine [PC 36:2], 1,2-dipalmitoyl-sn-glycero-phosphoethanolamine [PE 32:0] and N-palmitoyl-d-erythro-sphingosylphosphorylcholine [SM 34:1]) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Water (milliQ grade) was obtained from a Milli-Q integral water purification system (Millipore Corp., Burlington, MA, USA). For internal standards, SPLASH Lipidomix was purchased from Avanti Polar lipids (Alabaster, AL, USA).

1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine (OPC), bovine heart cardiolipin (CL), arachidonic acid (AA), hexane, hexadecane, KCl, HEPES, EDTA, Na₂SO₄, MES, Tris, adenosine 5-triphosphate (ATP), and guanosine 5-triphosphate (GTP) were purchased from Sigma-Aldrich (Munich, Germany). MPC (1-myristoyl-2-hydroxy-sn-glycero-3-phosphocholine) was obtained from Avanti Polar Lipids (Alabaster, AL, USA). Plain silica beads (22-μm diameter) were from Microspheres-Nanospheres (Corpuscular Co., Cold Spring, NY, USA). Chloroform was purchased from Carl Roth (Karlsruhe, Germany). 4-oxo-trans-2-nonenal (4-ONE) and 4-hydroxy-trans-2-nonenal (4-HNE) were purchased from Cayman Chemical (Ann Arbor, MI, USA) or synthesized as previously described [45 (link)].