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Anti cd4 bv605 gk1

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Anti-CD4 BV605 GK1.5 is a fluorochrome-conjugated antibody that binds to the CD4 antigen expressed on the surface of T helper cells. It is a tool used in flow cytometry applications to identify and quantify CD4+ T cells in biological samples.

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Lab products found in correlation

Live dead fixable aqua, by Thermo Fisher Scientific (1 mentions) Anti cd44 bv785 im7, by BioLegend (1 mentions) Streptavidin af647, by BioLegend (1 mentions) Anti cd73 apc, by BioLegend (1 mentions) Anti cd19 percp cy5, by BioLegend (1 mentions) Anti cd44 bv510, by BioLegend (1 mentions) Anti pd1 apc cy7, by BioLegend (1 mentions) Anti perforin apc, by BioLegend (1 mentions) Anti cd8 bv786, by BD (1 mentions) Anti cd69 bv650, by BioLegend (1 mentions) Anti ifn γ bv 421, by BioLegend (1 mentions) Anti cd107a pe, by BD (1 mentions)

Close spelling variants of this product

Anti-CD4 (GK1.5, Anti-CD4-BV605 CD4 (GK1.5, CD4 BV605 Anti-CD4

2 protocols using anti cd4 bv605 gk1

1

IL-7Rα Internalization Assay in T Cells

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IL-7Rα internalisation assay was performed similar as described before30 (link). In brief, CD4+ T cells were purified from the spleens of wild-type or T-Atg7−/− mice. After incubation with biotin-conjugated anti-IL-7Rα antibody (BioLegend, A7R34) 1:50 diluted in DPBS on ice for 30 min, cells were extensively washed and fractionated into the following groups (5 × 106 cells/group): 90 min on ice, 90, 60 and 30 min at 37 °C, 5% CO2. After incubation, cells were stained with AF647-Streptavidin (BioLegend, 1:1000), anti-CD4 BV605 GK1.5 (BioLegend, 1:400), anti-CD44 BV785 IM7 (BioLegend, 1:400), LIVE/DEAD Fixable Aqua (Invitrogen) stain for 20 min on ice and fixed with 2% PFA. The % internalised IL-7Rα is calculated as the Eq. (1) (MFI: mean fluorescence intensity): %internalised IL-7Rα=100−MFI sampleMFI90minice×100
Zhou D., Borsa M., Puleston D.J., Zellner S., Capera J., Sanderson S., Schifferer M., Hester S.S., Ge X., Fischer R., Jostins L., Behrends C., Alsaleh G, & Simon A.K. (2022). Mapping autophagosome contents identifies interleukin-7 receptor-α as a key cargo modulating CD4+ T cell proliferation. Nature Communications, 13, 5174.
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2

Multicolor Flow Cytometry Analysis of Liver and Spleen Lymphocytes

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Lymphocytes from the liver and spleen of mice were isolated as previously described23 (link) and analyzed by multicolor flow cytometry. Flow cytometry was performed on a CytoFLEX LX platform and results were analyzed using FlowJo software. The following antibodies were used in this study (clone, manufacture): anti-F4/80-Alexa Fluor 700 (BM8, BioLegend), anti-B220-Alexa Fluor 700 (RA3-6B2, BioLegend), anti-Cd11b-Alexa Fluor 700 (M1/70, BioLegend), anti-Cd3-Alexa Fluor 594 (17A2, BioLegend) anti-Cd4-BV605 (GK1.5, BioLegend), anti-Cd8-BV786 (53-6.7, BD), anti-Foxp3-BV421 (MF-14, BioLegend), anti-Cd62l-PerCP/Cy5.5 (MEL-14, BioLegend), anti-Cd44-BV510 (IM7, BioLegend), anti-NK1.1-BV510 (PK136, BioLegend), anti-A2Ar-FITC (7F6-G5-A2, NOVUS), anti-Cd73-APC (TY11.8, BioLegend), anti-Cd39-PECy7 (Duha59, BioLegend), anti-Cd19-PerCP Cy5.5(6D5, BioLegend), anti-PD1-APC/Cy7 (29F.1A12, BioLegend), anti-Cd107a- PE (1D4B, BD), anti-CD69-BV650 (H1.2F3, BioLegend), anti-Ifnγ-BV421 (XMG1.2, BioLegend), anti-Tnfα-PerCPCy5.5 (MP6-XT22, BioLegend), anti-GZMB-FITC (GB11, BioLegend), and anti-perforin-APC (S16009A, BioLegend).
Myojin Y., McCallen J.D., Ma C., Bauer K.C., Ruf B., Benmebarek M.R., Green B.L., Wabitsch S., McVey J.C., Fu C., Xie C, & Greten T.F. (2023). Adenosine A2a receptor inhibition increases the anti-tumor efficacy of anti-PD1 treatment in murine hepatobiliary cancers. JHEP Reports, 6(1), 100959.
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