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Hyaluronic acid methacrylate

Manufactured by Merck Group
Sourced in United States

Hyaluronic acid methacrylate is a synthetic compound used in the production of various types of lab equipment. It is a modified form of hyaluronic acid, a naturally occurring polysaccharide. The core function of hyaluronic acid methacrylate is to act as a structural component in the manufacturing of specialized equipment for scientific and medical applications.

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2 protocols using hyaluronic acid methacrylate

1

Hyaluronic Acid Hydrogel for Drug Delivery

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Poly(ethylene glycol) methyl ether methacrylate (OEGMA, average Mw = 300 g/mol), di(ethylene glycol) methyl ether methacrylate (MEO2MA), hyaluronic acid methacrylate (MeHA, degree of substitution: 20%-50%, MW 120,000-150,000), di(ethylene glycol) diacrylate (DEGDA 75.00%), hyaluronidase from bovine testes (Type I-S, 400-1000 units/mg, solid), potassium persulfate (KPS, 99,99%), sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), sodium chloride (NaCl), potassium chloride (KCl), monosodium phosphate (NaH2PO4) and disodium phosphate (Na2HPO4), were purchased from Sigma Aldrich (Saint Louis, Missouri, MO, USA). Doxorubicin hydrochloride (DOX) was purchased from LC Laboratories (Woburn, MA, USA). Mitoxantrone dihydrochloride (MTX) was purchased from MCE (NJ, USA). For the preparation of all solutions, deionized water (Hydrolab/HLP, Straszyn, Poland) with conductivity 0.055 μS·cm−1 was used.
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2

Multimodal Tracking of Endogenous Stem Cells

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Following our prior methods [[50] (link), [52] , [54] ], immunofluorescence was performed to image tissue sections using monoclonal antibodies and isotype-matched Alexa Fluor® secondary antibodies, with nucleus labeling with DAPI. All the tissue sections were made in 5-μm thickness, and the antigen retrieval procedures were performed following the manufacturer's protocols. CD44 (3660-CD-050, R&D Systems), lubricin (aa1151-1241, LSBio, Seattle WA), hyaluronic acid methacrylate (914800, SigmaAldrich), and/or type II collagen (COL-II) (C9301, Sigma-Aldrich) were co-labeled with multiple fluorescent secondary antibodies to track recruitment and differentiation of endogenous syMSCs. All primary antibodies and secondary antibodies were purchased from Abcam (Cambridge, MA), Santa Cruz Biotechnology (Dallas, Texas) or Life Technologies (Grand Island, NY). All images were acquired using an inverted fluorescence microscope (Olympus IX73, Waltham, MA).
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