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Gabpa sc 22810x

Manufactured by Santa Cruz Biotechnology
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GABPA (sc-22810X) is a primary antibody product available from Santa Cruz Biotechnology. It is a rabbit polyclonal antibody that recognizes the GABPA protein. GABPA is a transcription factor that plays a role in the regulation of gene expression.

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Lab products found in correlation

Creb1, by Cell Signaling Technology (2 mentions) Magna chip a g, by Merck Group (2 mentions) Hif 1α nb100 134, by Novus Biologicals (2 mentions)

2 protocols using gabpa sc 22810x

1

Chromatin Immunoprecipitation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed using 500 micrograms of chromatin. Chromatin was sonicated to fragments of ~500 bp and immunoprecipitated using 1 microgram of ChIP-grade antibodies: HIF1α (NB100–134, Novus Biological), GABPA (sc-22810X, Santa Cruz Biotechnology ), CREB1 (9104, Cell Signaling Technology) and with irrelevant IgG antibodies of mouse and rabbit origin and recovered using protein A/G magnetic beads (from Magna ChIP™ A/G, Millipore). The precipitated DNA was amplified by real-time qPCR, using primer sets designed to amplify regions of the target genes. ChIP primer sequences are provided in Supplementary Table 3:
Chhipa R.R., Fan Q., Anderson J., Muraleedharan R., Huang Y., Ciraolo G., Chen X., Waclaw R., Chow L.M., Khuchua Z., Kofron M., Weirauch M.T., Kendler A., McPherson C., Ratner N., Nakano I., Dasgupta N., Komurov K, & Dasgupta B. (2018). AMP Kinase Promotes Glioblastoma Bioenergetics and Tumor Growth. Nature cell biology, 20(7), 823-835.
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2

Chromatin Immunoprecipitation Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed using 500 micrograms of chromatin. Chromatin was sonicated to fragments of ~500 bp and immunoprecipitated using 1 microgram of ChIP-grade antibodies: HIF1α (NB100–134, Novus Biological), GABPA (sc-22810X, Santa Cruz Biotechnology ), CREB1 (9104, Cell Signaling Technology) and with irrelevant IgG antibodies of mouse and rabbit origin and recovered using protein A/G magnetic beads (from Magna ChIP™ A/G, Millipore). The precipitated DNA was amplified by real-time qPCR, using primer sets designed to amplify regions of the target genes. ChIP primer sequences are provided in Supplementary Table 3:
Chhipa R.R., Fan Q., Anderson J., Muraleedharan R., Huang Y., Ciraolo G., Chen X., Waclaw R., Chow L.M., Khuchua Z., Kofron M., Weirauch M.T., Kendler A., McPherson C., Ratner N., Nakano I., Dasgupta N., Komurov K, & Dasgupta B. (2018). AMP Kinase Promotes Glioblastoma Bioenergetics and Tumor Growth. Nature cell biology, 20(7), 823-835.
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