Human cxcl10 elisa kit

Plasma cytokines concentrations were determined using enzyme linked immunosorbent assays. Interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), macrophage inflammatory proteins 1 alpha (MIP-1α) and 1 beta (MIP-1β) were analyzed using a multiplex bead based immunoassay (MILLIPLEX^® MAP Human High Sensitivity T Cell Magnetic Bead Panel, Merck KgaA, Darmstadt, DE, USA). Interferon gamma induced protein 10 (IP-10) (Human CXCL10 ELISA kit, Abcam, Cambridge, UK) and soluble receptor of interleukin 2 (sCD25) were analyzed using the Human Quantikine Immunoassay (R&D Systems, Minneapolis, MN, USA). All followed the manufacturer’s instructions.

Plasma cytokine concentrations were measured in aliquot samples obtained at admission and stored at -80°C until analysis. IL-6, IL-8, IL-1β, TNF-α, interferon-gamma (IFN-γ), macrophage inflammatory proteins 1α (MIP-1α) and β (MIP-1β) were analyzed using a multiplex bead-based immunoassay (MILLIPLEX® MAP human high-sensitivity T cell magnetic bead panel). Interferon gamma-induced protein 10 (IP-10) and sCD25 were measured by the Human CXCL10 ELISA kit (Abcam, Cambridge, UK) and the Human Quantikine Immunoassay (R&D Systems), respectively. All samples were analyzed in duplicate and the median values were used as result for statistical analysis, unless the difference between the two determinations was greater than 30%, in which case the determinations were repeated.