The following antibodies were used in this study: anti-β-actin antibody (Sigma), human BMP-6 blocking antibody (R&D), anti-human BMP-6 detection antibody (Abcam, Cambridge, MA, USA), anti-human p65 antibody (Sigma), anti-human p50 antibody, anti-human p52 antibody (Cell Signaling), anti-Smad 5 antibody (Cell Signaling), and anti-phospho Smad 5 antibody (Cell Signaling).
Anti smad 5 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-Smad 5 antibody is a laboratory reagent used for the detection and analysis of the Smad 5 protein. Smad 5 is a member of the Smad family of proteins that play a crucial role in the transforming growth factor-beta (TGF-β) signaling pathway. This antibody can be utilized in various experimental techniques, such as western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of Smad 5 in biological samples.
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Lab products found in correlation
2 protocols using anti smad 5 antibody
1
Comprehensive Antibody Panel Analysis
2
Immunoprecipitation and Western Blot Analysis
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The C3H10T1/2 cells were washed and collected with cold PBS, lysed in cold lysis buffer containing 50 mM Tris (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 10% glycerol, 0.5 mM DTT, protease inhibitor cocktail tablets (EDTA-free) (Roche) and phosphatase inhibitor cocktail tablet (Roche). The cell lysates were precleared with IgG-agarose beads (Sigma) for 8 hr at 4°C.
IP was carried out by incubating the cell lysates with anti-Smad5 antibody (Cell Signaling), rabbit IgG immobilized on Protein G Plus-Agarose bead (Santa Cruz) at 4°C overnight. The immunocomplexes were pelleted and washed with cold lysis buffer six times. The proteins were released from beads by boiling in SDS sample buffer, and the samples were analyzed by western blotting.
IP was carried out by incubating the cell lysates with anti-Smad5 antibody (Cell Signaling), rabbit IgG immobilized on Protein G Plus-Agarose bead (Santa Cruz) at 4°C overnight. The immunocomplexes were pelleted and washed with cold lysis buffer six times. The proteins were released from beads by boiling in SDS sample buffer, and the samples were analyzed by western blotting.
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