Mouse anti cathepsin d

The following primary antibodies were employed for western blotting: mouse anti-β-tubulin (1:1000, cod. T5201; Sigma-Aldrich Corp.), mouse anti-β-actin (1:2000, cod. A5441; Sigma-Aldrich Corp.), rabbit anti-GAPDH (1:1000, cod. G9545; Sigma-Aldrich Corp.), mouse anti-cathepsin D (1:100, cod. IM03; Calbiochem, St. Louis, MO, USA), rabbit anti phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) (1:500, cod. 05-797R; Millipore, Burlington, MA, USA) and mouse anti-ERK1/2 (1:500, cod. 05-1152; Millipore). Secondary antibodies employed for immunoblotting were purchased as follows: Horse Radish Peroxidase-conjugated goat anti-mouse IgG (1:10,000, cod. 170–6516; Bio-Rad, Hercules, CA, USA) and Horse Radish Peroxidase-conjugated goat anti-rabbit IgG (1: 10,000, cod. 170–6515: Bio-Rad, Hercules, CA, USA). The following primary antibodies were employed for immunofluorescence: mouse anti-p21 (1:100, cod. sc-817; Santa Cruz Biotechnology, Dallas, TX, USA) and rabbit anti-Ki-67 (1:100, cod. HPA001164; Sigma-Aldrich). Secondary antibodies used for immunofluorescence were purchased as follows: goat-Anti Rabbit IgG Alexa FluorTM Plus 488 (1:1000, cod. A32731; Invitrogen) and Goat-Anti Mouse IgG Alexa FluorTM Plus 555 (1:1000, cod. A32727; Invitrogen).