Genomic DNA was extracted from the CF-CRC cells using the QIAamp DNA Blood midi kit (Qiagen, Hilden, Germany 51183), and fluorimetric quantification was performed (Qubit, Invitrogen, CA, USA). Proximal 5’-flanking, all exons and adjacent intronic zones, and the 3′-UTR of the CFTR gene (RefSeq NM_000492.4, NG_016465.4) were sequenced using the Sanger cycle sequencing protocol (ThermoFisher Scientific, Waltham, MA, USA), as previously described [31 –33 (link)] and a genetic analyzer (ABI PRISM 3130xl; Applied Biosystems, Foster City, CA, USA). Genotype analysis was completed using multiplex ligation-dependent probe amplification (SALSA MLPA probemix P091 CFTR, MRC Holland, Amsterdam, the Netherlands, EKI-FAM, P091-100).
Salsa mlpa probemix p091 cftr
Manufactured by MRC-Holland
Sourced in Netherlands
The SALSA MLPA probemix P091 CFTR is a laboratory equipment product designed for the detection and analysis of genetic variations in the CFTR gene. It provides a comprehensive and efficient method for the identification of deletions and duplications within the CFTR gene, which is associated with cystic fibrosis. The product is suitable for use in genetic research and diagnostic applications.
Automatically generated - may contain errors
4 protocols using salsa mlpa probemix p091 cftr
1
CFTR Gene Sequencing and Genotyping
2
CFTR Gene Sequencing and Genotyping
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Genomic DNA was extracted from the CF-CRC cells using the QIAamp DNA Blood midi kit (Qiagen, Hilden, Germany 51183), and fluorimetric quantification was performed (Qubit, Invitrogen, CA, USA). Proximal 5’-flanking, all exons and adjacent intronic zones, and the 3′-UTR of the CFTR gene (RefSeq NM_000492.4, NG_016465.4) were sequenced using the Sanger cycle sequencing protocol (ThermoFisher Scientific, Waltham, MA, USA), as previously described [31 –33 (link)] and a genetic analyzer (ABI PRISM 3130xl; Applied Biosystems, Foster City, CA, USA). Genotype analysis was completed using multiplex ligation-dependent probe amplification (SALSA MLPA probemix P091 CFTR, MRC Holland, Amsterdam, the Netherlands, EKI-FAM, P091-100).
3
CFTR Gene Sequencing and Genotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA was extracted from the CF-CRC cells using the QIAamp DNA Blood midi kit (Qiagen, Hilden, Germany 51183), and fluorimetric quantification was performed (Qubit, Invitrogen, CA, USA). Proximal 5’-flanking, all exons and adjacent intronic zones, and the 3′-UTR of the CFTR gene (RefSeq NM_000492.4, NG_016465.4) were sequenced using the Sanger cycle sequencing protocol (ThermoFisher Scientific, Waltham, MA, USA), as previously described [31 –33 (link)] and a genetic analyzer (ABI PRISM 3130xl; Applied Biosystems, Foster City, CA, USA). Genotype analysis was completed using multiplex ligation-dependent probe amplification (SALSA MLPA probemix P091 CFTR, MRC Holland, Amsterdam, the Netherlands, EKI-FAM, P091-100).
4
CFTR Gene Sequencing and Analysis
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Genomic DNA was extracted from both the peripheral blood leucocytes of enrolled individuals and derived undifferentiated CF-CRC-AESC, using the QIAamp DNA Blood midi kit (Qiagen, Hilden, Germany) and quantified using fluorimetry (Qubit, Invitrogen, CA, USA). The proximal 5′-flanking, all exons and adjacent intronic zones, and the 3′-UTR of the CFTR gene (RefSeq NM_000492.4, NG_016465.4) were sequenced by a Sanger cycle sequencing protocol (ThermoFisher Scientific, Waltham, MA, USA) as previously described [21 (link),22 (link),23 (link)], using a genetic analyzer (ABI PRISM 3130xl; ThermoFisher Scientific). Genetic analysis was completed through multiplex ligation-dependent probe amplification (SALSA MLPA probemix P091 CFTR, MRC Holland, Amsterdam, The Netherlands) to unveil macrodeletions/macroduplications. The genotype of derived undifferentiated CF-CRC-AESC was confirmed using the same methodology. Results of mutational analysis have already been published [5 (link),19 (link)].
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