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Human cot 1 dna

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Human Cot-1 DNA is a laboratory product used for various research applications. It is a type of repetitive DNA sequence found in the human genome, which is often used as a probe or reference material in genetic studies and assays. The core function of Human Cot-1 DNA is to provide a standardized and well-characterized DNA sample for scientific investigations, without any further interpretation or extrapolation on its intended use.

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Lab products found in correlation

H 3300, by Vector Laboratories (2 mentions) Ab53494, by Abcam (2 mentions) Duolink probe anti mouse minus, by Merck Group (2 mentions) Mouse h3k4me2, by Merck Group (2 mentions)

2 protocols using human cot 1 dna

1

Chromatin Immunoprecipitation for H3K4me2 in Human Coronary Lesions

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Paraffin-embedded Human coronary lesions were deparaffinized in xylene and rehydrated in ethanol. Citrate-based antigen retrieval was performed as described by the manufacturer (H-3300 Vector Laboratories). Slides were blocked for 1 hour at room temperature with a mixture of PBS, fish skin gelatin, and 10% horse serum. ISH-PLA staining for H3K4me2 at the Myh11 promoter was performed7 using Duolink Probe anti-mouse MINUS (Millipore Sigma DUO92004) and anti-rabbit PLUS (Millipore Sigma DUO92002) and In Situ Orange (Millipore Sigma DUO92007). Antibodies were as follows: mouse H3K4me2 (Millipore Sigma 05–1338, 100 μg, clone CMA303, 1:100), rabbit Biotin (Abcam ab53494, 1 mg/mL, 1:100), ACTA2 was conjugated to -FITC (Sigma F3777 clone 1A4, 1:500). Nuclear counterstain was performed with DAPI containing mounting media (DUO82040 Sigma-Aldrich Duolink In Situ Mounting Medium with DAPI). Notes important to hybridization: Pepsin solution pH=4.3. Human Cot-1 DNA was from Millipore Sigma (11581074001 Roche).
Newman A.A., Serbulea V., Baylis R.A., Shankman L.S., Bradley X., Alencar G.F., Owsiany K., Deaton R.A., Karnewar S., Shamsuzzaman S., Salamon A., Reddy M.S., Guo L., Finn A., Virmani R., Cherepanova O.A, & Owens G.K. (2021). Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms. Nature metabolism, 3(2), 166-181.
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2

Chromatin Immunoprecipitation for H3K4me2 in Human Coronary Lesions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paraffin-embedded Human coronary lesions were deparaffinized in xylene and rehydrated in ethanol. Citrate-based antigen retrieval was performed as described by the manufacturer (H-3300 Vector Laboratories). Slides were blocked for 1 hour at room temperature with a mixture of PBS, fish skin gelatin, and 10% horse serum. ISH-PLA staining for H3K4me2 at the Myh11 promoter was performed7 using Duolink Probe anti-mouse MINUS (Millipore Sigma DUO92004) and anti-rabbit PLUS (Millipore Sigma DUO92002) and In Situ Orange (Millipore Sigma DUO92007). Antibodies were as follows: mouse H3K4me2 (Millipore Sigma 05–1338, 100 μg, clone CMA303, 1:100), rabbit Biotin (Abcam ab53494, 1 mg/mL, 1:100), ACTA2 was conjugated to -FITC (Sigma F3777 clone 1A4, 1:500). Nuclear counterstain was performed with DAPI containing mounting media (DUO82040 Sigma-Aldrich Duolink In Situ Mounting Medium with DAPI). Notes important to hybridization: Pepsin solution pH=4.3. Human Cot-1 DNA was from Millipore Sigma (11581074001 Roche).
Newman A.A., Serbulea V., Baylis R.A., Shankman L.S., Bradley X., Alencar G.F., Owsiany K., Deaton R.A., Karnewar S., Shamsuzzaman S., Salamon A., Reddy M.S., Guo L., Finn A., Virmani R., Cherepanova O.A, & Owens G.K. (2021). Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms. Nature metabolism, 3(2), 166-181.
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