Vero cell line (ATCC, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM; 8118305, GIBCO/Thermo Fisher Scientific, USA) with 10% fetal bovine serum (FND500, ExCell Bio, Shanghai, China). The neuroblastoma cell line SK-N-SH (ATCC, HTB-11, American Type Culture Collection, Manassas, VA, USA) was propagated in Eagle’s minimal essential medium (MEM; GIBCO /Thermo Fisher Scientific, USA) supplemented with 10% FBS. In our previous studies, HSV-1 infection induced the biphasic F-actin dynamics in SK-N-SH cells [26 (link)]. In this study, the SK-N-SH cells were used to study the effect of amentoflavone on F-actin. HSV-1 strain F (ATCC, USA), initially obtained from Hong Kong University, was propagated in Vero cells and stored at −80 °C until use. HSV-1/Blue, a TK mutant derived from HSV-1 (KOS) and two acyclovir-resistant clinical HSV-1 strains HSV-1/106 and HSV-1/153 were kind gifts from Tao Peng, State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences. GFP-HSV-1, expressing a GFP-tagged viral protein Us11, was used to evaluate viral nuclear transport [27 (link)]. HSV-1 Us11 is a multifunctional late protein which can regulate the accumulation of RNA species and facilitate HSV-1 replication [28 (link)].
Fnd500
Manufactured by ExCell Bio
Sourced in China, United States, Australia
The FND500 is a high-performance fluorescence nanodiamond detector designed for advanced applications in materials science and biological research. The device utilizes state-of-the-art optical and electronic components to accurately measure the fluorescence properties of nanodiamond samples. Its core function is to provide reliable and precise data on the optical characteristics of nanodiamond materials.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Hsv 1 strain f, by American Type Culture Collection (5 mentions)
M3148, by Merck Group (4 mentions)
N1250, by Solarbio (4 mentions)
Sh sy5y, by American Type Culture Collection (3 mentions)
Vero cell, by American Type Culture Collection (3 mentions)
Hacat, by American Type Culture Collection (3 mentions)
Vero cell line, by American Type Culture Collection (2 mentions)
Lif1010, by Merck Group (2 mentions)
Ccd 18co, by American Type Culture Collection (2 mentions)
Sk n sh, by American Type Culture Collection (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Sartorius (1 mentions)
P1400, by Solarbio (1 mentions)
Enzalutamide, by Merck Group (1 mentions)
Mouse b cell isolation kit, by STEMCELL (1 mentions)
L2630, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
24 protocols using fnd500
1
Investigating HSV-1 Dynamics in Neuroblastoma
2
Cell Culture Protocols for HEK293T, HeLa, and GSC Lines
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Human HEK293T cells purchased from the ATCC, and stat3-null HeLa cells purchased from ABclonal (20170728-02) were grown in Dulbecco's modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS; FND500, excellbio), 100 U/ml penicillin and 100 U/ml streptomycin. GSC lines were obtained from Professor Jeremy N. Rich (University of California San Diego). GSCs were cultured in Neurobasal™ medium (12348017, Gibco) supplied with B27 supplement (12587010, Gibco), 10 ng/ml epidermal growth factor (EGF) and 10 ng/ml fibroblast growth factor (FGF) as previously described (17 (link),18 (link)). All cell lines were used for a limited number of passages and checked for Mycoplasma infection.
3
Culture of Mouse ESCs and Human iPSCs
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The 46C mouse ESCs were kindly provided by Qi-Long Ying (The University of Southern California) and were cultured in 0.1% gelatin-coated dishes at 37 °C in 5% CO2. The conventional cell culture conditions were Dulbecco's modified Eagle's medium (Biological Industries) supplemented with 10% fetal bovine serum (FND500, ExCell Bio), 1× MEM nonessential amino acids (N1250, Solarbio), 1× penicillin/streptomycin (P1400, Solarbio), 0.1 mM β-mercaptoethanol (M3148, Sigma), and LIF (made in house). Human transgene-free iPSCs were kindly provided by Nuwacell Ltd (ZSSY-001) and were cultured in ncTarget (RP01020, Nuwacell Ltd). Cells were dissociated using EDTA solution (RP01007, Nuwacell Ltd) every 3 to 5 days.
4
Generation of Enzalutamide-Resistant PCa Cells
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Seven PCa cell lines were used in this research, including LNCaP, LNCaP-AI, C4-2, C4-2-EnzR, PC-3, DU145, and 22RV1. LNCaP, C4-2, PC-3, DU145, and 22RV1 were cultured in RPMI-1640 medium (BI, 01-100-1ACS, USA) supplemented with 10% fetal bovine serum (ExCell Bio, FND500, New Zealand). LNCaP-AI was generated in our previous research and was cultured in RPMI-1640 medium (BI, 01-100-1ACS, USA) supplemented with 10% certified charcoal stripped foetal bovine serum (BI, 04-201-1A, USA). As for C4-2-EnzR cell generation, C4-2 cells were first cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1 μM enzalutamide (Sigma, 532996, Germany). After the cell growth was not restricted, we increased the enzalutamide concentration gradually at 2, 5, 10, and 20 μM. When C4-2 cells can grow stably in the medium containing 20 μM enzalutamide, we maintain this concentration and pass it 50 times, and consider this cell as an enzalutamide-resistant C4-2 cell (C4-2-EnzR).
5
Vero and SH-SY5Y Cell Culture for HSV-1
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Vero cell line (ATCC, Manassas, VA, USA) was cultured in Dulbecco’s modified Eagle’s medium (DMEM; 8118305, GIBCO/Thermo Fisher Scientific, Gaithersburg, MD, USA) with 10% fetal bovine serum (11011-8611, TIANHANG, Hangzhou, China). SH-SY5Y (ATCC, Manassas, VA, USA), a human neuroblastoma cell line was cultured in Dulbecco’s modified Eagle’s medium (DMEM; 8118305, GIBCO/Thermo Fisher Scientific, Gaithersburg, MD, USA) with 10% fetal bovine serum (FND500, ExCell Bio, Shanghai, China). HSV-1 strain F (ATCC, Manassas, VA, USA), initially obtained from Hong Kong University, was propagated in Vero cells and stored at −80 °C until use. EGFP-HSV-1, expressing an EGFP-tagged viral protein Us11, a kind gift from the College of Pharmacy, Jinan University (Guangzhou, China), was used to evaluate viral replication capacity [18 (link)].
6
Culturing Murine Colon and Human Kidney Cells
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The MC38 cell line derived from C57BL/6 murine colon adenocarcinoma cells was obtained from Professor Yangxin Fu of the University of Texas, Southwestern Medical Center. The HEK293T cell line was obtained from Professor Ping Gao of South China University of Technology. These two cell lines were both cultured in DMEM medium (SH30243.FS, HyClone, USA) containing 10% fetal bovine serum (FBS) (FND500, ExCell Bio, China) at 37 °C in a 5% CO2 atmosphere. All cells used in this study were mycoplasma-free.
7
DLBCL Cell Culture Protocol
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Human DLBCL cells: we chose the OCI-LY1 cell line (GCB) in the Institute of Jennio Biosciences (CBP60265, Guangzhou, China) and cultured them within RPMI-1640 medium (C11875500CP/8119428, Gibco, USA) that contained 1% penicillin-streptomycin (PS) (15070-063, 10000U, GIBCO, USA) as well as 10% fetal bovine serum (FBS) (FND500, Excell Bio, USA). All cells were incubated at 37°C in a humidified incubator with 5% CO2.
8
Established Cell Lines and Virus Stocks
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African green monkey kidney cell line (Vero cells) and Human immortalized keratinocyte cell line (HaCaT) were purchased from the American Type Culture Collection Center (ATCC). Vero, HaCaT, SH-SY5Y (ATCC CRL-2266), and BV2 (Cell Bank, Chinese Academy of Sciences) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 8118305, GIBCO/Thermo Fisher Scientific, United States) with 10% fetal bovine serum (FND500, ExCell Bio, Shanghai, China). HSV-1 F strain (ATCC, United States) preserved by the University of Hong Kong, was propagated in Vero cells and stored in the refrigerator at −80°C. HSV-1/Blue, a TK mutant derived from HSV-1 KOS strain and two clinical ACV-resistant HSV-1 strain, HSV-1/106 and HSV-1/153, were kind gifts from Prof Tao Peng (Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, China). Green fluorescent protein (GFP)-tagged HSV-1 F strain (GFP-HSV-1) (GFP-tagged viral protein Us11) was obtained from the research group of Professor Yuan Li (Jinan University, China).
9
Splenic Naive B Cell Differentiation
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Splenic naive B cells were purified with a mouse B-cell isolation kit from Stemcell (#19854) and the purified naive B cells were cultured at a density of 5 × 105 cells ml−1 in RPMI medium supplemented with 15% FBS (FND500, ExCell Bio), 31.25 g ml−1 LPS (L2630, Sigma) and 25 ng ml−1 of IL4 (CK15; Novoprotein), or 31.25 g ml−1 LPS alone. Cells were collected every day up to day 4 and were stained with surface markers to access the antibody class. The flow cytometric data were analyzed with FlowJo X 10.0.7R2. The gate strategies are showed in Supplementary Fig. 14 .
10
Cell Seeding and Culture Conditions
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PK15, IPEC-J2, and 3D4/21 cells were seeded at the concentration of 1 × 105 cells per well in 12 well plates containing 1 mL of DMEM (Gibco, 12800082) or RPMI-1640 (Gibco, 42401042), respectively, and supplemented with 10% fetal bovine serum (ExCell Bio, FND500). Cells were cultured in a highly humidified atmosphere of 95% air and 5% CO2 at 37°C.
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