Cal27, SCC-25, and SCC-15 cell lines, sourced from the American Type Culture Collection (ATCC, Manassas, VA), were utilized in our study. Cal27 cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, ATCC), while SCC-15 and SCC-25 cells were maintained in MEM NEAA medium. All cell cultures were incubated at 37 °C in a 5% CO2 atmosphere. Antibiotics used included a penicillin-streptomycin-gentamicin mix (100×, P1410-100 ml) and a penicillin-streptomycin solution (100×, P1400-100 ml) to prevent contamination.
Scc25
Manufactured by American Type Culture Collection
Sourced in United States, China, Japan, United Kingdom
The SCC25 is a laboratory equipment product designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cell lines. The device offers temperature, humidity, and CO2 regulation to support optimal cell growth conditions.
Automatically generated - may contain errors
Lab products found in correlation
Cal27, by American Type Culture Collection (151 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (78 mentions)
Scc15, by American Type Culture Collection (72 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (71 mentions)
Scc25, by Thermo Fisher Scientific (49 mentions)
Dmem f12, by Thermo Fisher Scientific (45 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (24 mentions)
Cal27, by Thermo Fisher Scientific (23 mentions)
Penicillin, by Thermo Fisher Scientific (20 mentions)
Hydrocortisone, by Merck Group (19 mentions)
Streptomycin, by Thermo Fisher Scientific (19 mentions)
Scc 4, by American Type Culture Collection (17 mentions)
Scc15, by Thermo Fisher Scientific (13 mentions)
Hsc 3, by American Type Culture Collection (12 mentions)
Rpmi 1640, by Thermo Fisher Scientific (11 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (9 mentions)
Scc 4, by Thermo Fisher Scientific (8 mentions)
Scc090, by American Type Culture Collection (6 mentions)
Hsc 3, by Thermo Fisher Scientific (6 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
293 protocols using scc25
1
Oral Squamous Cell Carcinoma Cell Lines
2
Establishing Human Oral Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SAS (JCRB0260; JCRB), SCC25 (CRL-1628; ATCC), and HSC3 cells are the human oral squamous cell carcinoma cell line. The SAS cell line was provided to us by Dr. Lo (Institute of Oral Biology, Department of Dentistry, National Yang-Ming University, Taipei, Taiwan). The SCC25 cell line was obtained from the American Type Culture Collection (ATCC). The HSC-3 cell line was provided to us by Dr. Yeh (Department of Hematology and Oncology, Cancer Center, Taipei Medical University). All the cell lines were cultured in RPMI 1640 (GIBCO, USA) supplemented with 10% fetal bovine serum (BI, USA), 1% penicillin/streptomycin, and 2 mmol/L L-glutamine in a humidified incubator with a 5% CO2 at 37°C.
3
Maintenance and Characterization of HNSCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HNSCC cell lines (JHU-011, JHU-022 and JHU-029) we established at Johns Hopkins University, and were maintained in the RPMI medium containing 10% Fetal Bovine Serum (FBS), penicillin (100 units/mL) and streptomycin (100 μg/mL). SCC25, FaDu, Cal27, SCC25, and SCC22b cell lines were obtained from ATCC and grown in the recommended medium. Tert-1 immortalized normal human oral mucosal keratinocytes cell line OKF6 was received from Dr. James Rhienwald, Harvard Medical School, and spontaneously immortalized NOK-SI cell line was received from Dr. Silvio Gutkind, National Institute of Dental and Craniofacial Research. Cells were maintained in Keratinocyte-SFM (Thermo Scientific, Cat# 17005042). The cells were periodically monitored for mycoplasma using the MycoDtect kit (Greiner Bio-One). All experiments were performed within 6 months of the mycoplasma screen. All cells were incubated at 37°C with 5% CO2.
4
Oral Squamous Cell Carcinoma Cell Lines and Oral Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
OSCC cell lines CAL27, SCC4 and SCC25 were acquired from the American Tissue Culture Collection (ATCC), certified as mycoplasma-free and STR-authenticated. The cell lines were grown in 5% CO2 at 37°C in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2.5 mM L-glutamine (CAL27) or a 1:1 mixture of DMEM and Ham’s F12 medium containing 2.5 mM L-glutamine, 400 ng/ml hydrocortisone and 10% FBS (SCC4 and SCC25).
Bacterial strains S. mitis ATCC 49456, N. flavescens ATCC 13120 and P. gingivalis ATCC 33277 were obtained from ATCC whereas H. parainfluenzae NCTC 10665 was obtained from Public Health England. P. gingivalis was included as a pathogenic control given existing evidence on its carcinogencity [2 ,3 (link)]. Brain Heart Infusion (BHI) supplemented with 0.5% hemin, 0.1% Vitamin K and 1% Isovitalex was used as a culture medium for all bacteria. The health-associated strains were grown at 37°C in 5% CO2; P. gingivalis was grown at 37°C in anaerobic conditions (10% hydrogen, 10% CO2, and 80% nitrogen).
Bacterial strains S. mitis ATCC 49456, N. flavescens ATCC 13120 and P. gingivalis ATCC 33277 were obtained from ATCC whereas H. parainfluenzae NCTC 10665 was obtained from Public Health England. P. gingivalis was included as a pathogenic control given existing evidence on its carcinogencity [2 ,3 (link)]. Brain Heart Infusion (BHI) supplemented with 0.5% hemin, 0.1% Vitamin K and 1% Isovitalex was used as a culture medium for all bacteria. The health-associated strains were grown at 37°C in 5% CO2; P. gingivalis was grown at 37°C in anaerobic conditions (10% hydrogen, 10% CO2, and 80% nitrogen).
5
Acquisition and Maintenance of HNSCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HNSCC cell lines SCC9, SCC15, SCC25, CAL27 and HEK293T cells were obtained from ATCC (Rockville, MD, USA). UM1 and UM-SCC1 was provided by Dr. Xiaofeng Zhou (University of Illinois at Chicago, IL, USA). HSC3, HSC6 and CAL33 were kindly provided by J. Silvio Gutkind (NIH, Bethesda, MD, USA). The characteristics of HNSCC cell lines are described in Table S1 . The UM-SCC1, HSC3, HSC6, CAL27, CAL33 and HEK293T cells were cultivated in Dulbecco's modified Eagle's medium (DMEM, Gibco, Rockville, MD, USA) supplemented with 10% fetal bovine serum (FBS, Gibco). The SCC9, SCC15, SCC25 and UM1 cells were maintained in DMEM-F12 (Gibco) supplemented with 10% FBS. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO2. All cell lines were routinely tested for Mycoplasma by PlasmoTestTM Mycoplasma contamination detection kit (InvivoGen).
6
Establishment and Authentication of HNSCC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human HNSCC cell lines FaDu, Cal27, SCC4, SCC9 and SCC25 were purchased from ATCC (https://www.lgcstandards-atcc.org ). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% foetal bovine serum (Invitrogen, Germany), 2 mM l -glutamine and 50 μg/ml penicillin-streptomycin in humidified and sterile conditions with 6% CO2 at 37 °C. Cell cultures were regularly screened to exclude mycoplasma contamination (Venor®GeM Classic Mycoplasma Detection Kit, Minerva Biolabs) according to manufacturer’s recommendation, and the authentication of all cell lines was confirmed by the Multiplex Human Cell Line Authentication Test (Multiplexion, Germany, latest update April 2019).
7
Cell Lines for HNSCC and Oral Cancer Research
8
Culturing Oral Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human normal oral epithelial cells (HNOECs), SCC-15, sCC-25, and CAL-27 cells were cultured in complete medium (DMED [Gibco]) containing 10% fetal bovine serum (FBS, Invitrogen, California, USA), penicillin, and streptomycin (Solarbio, Beijing, China) at 37°C in 5% CO2, 95% air. HNOECs were purchased from Guide Chem (https://china.guidechem.com , Zhejiang, China) and SCC-15, sCC-25, and CAL-27 cells were obtained from ATCC (www.atcc.org , USA).
9
HIOEC and OSCC Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HIOECs were kindly donated by Professor Cheng-zhang Li and cultured in KGM-gold (Lonza, Switzerland) supplemented with growth factors. The human OSCC cell lines SCC25 and CAL27 were purchased from ATCC (Manassas, US). Ten percent FBS (Gibco, USA) was added to the medium of SCC25 and CAL27 cells. All cells were cultured in a constant temperature and pressure incubator at 37 °C and 5% CO2.
10
Cell Line Maintenance and Identification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAL-27, SCC-4, SCC-9 and SCC-25 cell lines were purchased from the ATCC and maintained according to the ATCC recommendations. WSU-HN-4, HN-6, HN-13 cell lines and normal epithelial cells (NECs) were acquired and cultured as well as genetic identified as described in the previous study [18 (link), 19 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!