BCL11A expression was examined in two NSCLC cell lines [NCI-H1703 (ATCC, Manassas, VA, USA; CRL-5889), A549 (ATCC; CCL-185)] and normal lung fibroblast cell line (IMR-90) (ATCC; CCL-186). NCI-H1703 was derived from stage I SCC, while A549 was the AC cell line. IMR-90 was cultured in Minimum Essential Medium supplemented with non-essential amino acids (Sigma-Aldrich, Saint Louis, MO, USA). RPMI-1640 (Lonza, Basel, Switzerland) was used to culture NCI-H1703, while A549 was grown in Kaighn’s Modification of Ham’s F12 Medium (ATCC). All culture media were supplemented with 10% fetal bovine serum, L-glutamine (2 mM) and a 1% mixture of antibiotics (100 U/mL penicillin G and 100 µg/mL streptomycin). The reagents were obtained from Sigma-Aldrich. The cells were grown under constant conditions (37 °C in an incubator, 5% CO2, 95% humidity) (HERAcell 150i, Thermo Fisher).
Nci h1703
Manufactured by American Type Culture Collection
Sourced in United States
NCI-H1703 is a human non-small cell lung carcinoma cell line derived from a male patient. It is a commonly used model for studying lung cancer biology and drug development.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (13 mentions)
Nci h460, by American Type Culture Collection (12 mentions)
Beas 2b, by American Type Culture Collection (12 mentions)
Nci h520, by American Type Culture Collection (10 mentions)
Nci h1299, by American Type Culture Collection (8 mentions)
Sk mes 1, by American Type Culture Collection (7 mentions)
Nci h522, by American Type Culture Collection (7 mentions)
Nci h1975, by American Type Culture Collection (7 mentions)
Hcc827, by American Type Culture Collection (6 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
Nci h226, by American Type Culture Collection (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Nci h358, by American Type Culture Collection (4 mentions)
Nci h1703, by Thermo Fisher Scientific (4 mentions)
Rpmi 1640 medium, by American Type Culture Collection (3 mentions)
Nci h2170, by American Type Culture Collection (3 mentions)
Nci h1703, by Lonza (3 mentions)
Dms114, by American Type Culture Collection (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
45 protocols using nci h1703
1
BCL11A Expression in NSCLC Cells
2
Cell Line Maintenance and Inhibitor Evaluation
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DMS114, NCI-H1581, NCI-H1703, NCI-H2170, and NCI-H520 cell lines were obtained from ATCC. NCI-H1581 cells were routinely maintained in DMEM/F12; DMS114 in Waymouth’s MB752/1; whereas NCI-H1703, NCI-H2170, and NCI-H520 were maintained in RPMI 1640 medium. All culture media contained 10% of FBS and penicillin/streptomycin (100 U/mL/100 μg/mL). Cells were grown at 37 °C in a humidified atmosphere of 5% CO2. All culture media and corresponding supplements were purchased from Merck KGaA (Darmstadt, Germany) or Biowest (Riverside, MO, USA). CPL304110 (WO/2014/141015) inhibitor was provided by Celon Pharma S.A., Poland [17 (link)]. SB202190 and SB203580 inhibitors were purchased from Selleckchem (Houston, TX, USA).
3
Cell Culture Protocols for Cancer Research
4
NSCLC Tumor and Non-Tumor Tissue Collection
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NSCLC tumor and paired non-tumor tissue samples were collected from all patients. Tumor tissues were obtained from the central part of the lesion. The adjacent non-tumor tissues were more than 5 cm away from the tumor lesion. Non-tumor tissues were macroscopically and microscopically confirmed to be not invaded by cancer cells. Two NSCLC cell lines HCC4006 (LUAD) and NCI-H1703 (LUSC) (ATCC, USA) and normal human lung epithelial cells BEAS-2B (SCSP-5067, Cell Bank of Type Culture Collection of Chinese Academy of Sciences, Shanghai, China) were used in the study and cultured in media containing 10% FBS and 90% DMEM (GIBCO, California, USA) at 37°C in a humidified incubator with 5% CO2.
5
Cell Line Maintenance and Validation
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HEK293T cells (American Type Culture Collection [ATCC]; catalog #ACS-4500) and the NSCLC cell lines NCI-H1703 (ATCC; catalog #CRL-5889) and NCI-H1437 (ATCC; catalog CRL-5872) were purchased from ATCC. HEK293T, NCI-H1703, and NCI-H1437 cells were maintained in RPMI 1640 (HyClone; #SH30027.01) medium containing 10% FBS (Thermo Fisher Scientific #10099141) and penicillin/streptomycin (Thermo Fisher Scientific; #15140122). All these cells were cultured at 37°C in a 5% CO2 humidified atmosphere. None of the cell lines in this study appeared in the misidentified cell line list maintained by the International Cell Line Authentication Committee. All cell lines were routinely tested for microbial contamination (including mycoplasma).
6
Mesothelioma and Lung Cancer Cell Line Culture
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Mesothelioma (MERO-14, MERO-41, MERO-82, NO36, ONE58, MSTO-211H, NCI-H226, NCI-H2052) and lung cancer (NCI-H1703, -H211, -H526, -H522, -H520, -H460, -H358, -H1395, -H2122, -H2023, -H1651, A549, SW1573, DMS-114, DMS-53) cell lines were obtained from ATCC (Manassas, VA), DSMZ (Braunschweig, Germany), or Sigma-Aldrich (St. Louis, MO). Cells were cultured in the appropriate culture medium supplemented with 10% fetal bovine serum (FBS) at 37°C in humidified incubators under 5% CO2. Heparin sodium salt and growth factors were purchased from Sigma-Aldrich. GSK3052230 is formulated in 0.94 mg/mL sodium phosphate monobasic, 1.9 mg/mL sodium phosphate dibasic, 8.8 mg/mL sodium chloride, 0.2 mg/mL polysorbate 80, pH 7.0 at a stock concentration of 12.8 mg/mL. NVP-BGJ398 was purchased from Selleck Chemicals (Houston, TX) and dissolved in DMSO at a stock concentration of 20 mM.
7
Characterization of Lung Cancer Cell Lines
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Human lung adenocarcinoma A549 cells (#CCL-185, passage number 1-12) and human lung squamous cell carcinoma NCI-H1703 [H1703] cells (#CRL-5889, passage number 1-12) were purchased from ATCC (Manassas, VA, USA). A549 clonal cell lines, 1-40 and 2-11, were obtained from Bialk et al [6 (link)]. Cells were thawed, according to the manufacturer’s protocol. A549 cells were grown in F-12K medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC). NCI-H1703 cells were grown in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum (FBS) (ATCC). Both cell lines were grown at 37 °C in 5% CO2. Cell lines were tested for Mycoplasma upon thawing and before use in experiments using the MycoScope PCR Mycoplasma detection kit (Genlantis, Cat. MY01100).
8
Modulating miR-1306 in Lung Cancer
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LUSC cell lines (NCI-H1703, NCI-H2170, NCI-H520, SK-MES-1) and the normal BEAS-2B cells were purchased from ATCC. Cells were cultured in the DMEM culture medium (Invitrogen, USA) at 37°C with 5% CO2. Cultured cells were transfected with miR-1306 mimic or inhibitor, or their corresponding negative controls with the help of Lipofectamine 3000 Transfection reagent (Invitrogen, USA). The efficiency of cell transfection was evaluated by PCR.
9
Cell Culture Protocols for Cancer Research
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U-2 OS T-Rex Flp-In cells, a gift from Dr. Daniel Durocher (The Lunenfeld-Tanenbaum Research Institute), and U-2 OS Tet-On cells (Clontech) were cultured in DMEM containing 4.5 g/L glucose and L-glutamine (Corning). NCI-H460, NCI-H2291, NCI-H1703 and NCI-H446 cells (ATCC) were cultured in RPMI1640 containing high glucose, L-glutamine and HEPES (ATCC). U-2 OS COQ2 knockout cells were grown in DMEM supplemented with 200 μM uridine and FSP1/COQ2 double knockout cells were grown in DMEM supplemented with 200 μM uridine and 1 μg/mL Ferrostatin-1. NCI-H460 GPX4 knockout lines and FSP1/GPX4 double knockout lines were grown in RPMI1640 supplemented with 1 μg/mL Ferrostatin-1. All media was supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific and Gemini Bio Products), and all cell lines were grown at 37°C with 5% CO2. All cell lines were tested for mycoplasma and were not authenticated.
10
Lung Cancer Cell Line Sensitivity to Dasatinib and Rapamycin
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The human adenocarcinoma cell line A549, human lung squamous carcinoma cell line NCI-H1703, human large-cell lung cancer cell line NCI-H460 and normal bronchial epithelial cell BEAS-2B were purchased from ATCC (Beijing, China), and cultured in dulbecco's modified eagle medium (Life technology, Shanghai, China) supplemented with 10% fetal bovine serum, at 37°C in a humidified atmosphere with 5% CO2. Cell number and viability were determined by trypan blue exclusion, with at least 90% viable cells before treatment exposure. A549 cells were treated with Dasatinib (5, 10, 25, or 50 nM) alone or in combination with Rapamycin (20, 50, or 100 nM) for 24 to 96 hours. Cells treated with 0.1% dimethylsulfoxide (DMSO) were used as vehicle control.
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