The human SK-N-SH (ATCC HTB-11) neuroblastoma line was obtained from the American Type Culture Collection. Cells were routinely cultured with F12-DMEM (Gibco-BRL) media containing 10% fetal calf serum (FCS), 1% penicillin and streptomycin, and 0.1% gentamicin at 37°C, 5% CO2.
Sk n sh atcc htb 11
Manufactured by American Type Culture Collection
Sourced in United States
SK-N-SH (ATCC HTB-11) is a human neuroblastoma cell line derived from a bone marrow biopsy. The cell line is suitable for various cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Calmodulin, by Cell Signaling Technology (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
α synuclein, by Cell Signaling Technology (1 mentions)
Forskolin, by Merck Group (1 mentions)
Western bright peroxide, by Advansta (1 mentions)
Vamp2, by Cell Signaling Technology (1 mentions)
Synaptotagmin 1, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Westernbright ecl, by Advansta (1 mentions)
Minimal essential media, by Merck Group (1 mentions)
3 protocols using sk n sh atcc htb 11
1
Culturing SK-N-SH Neuroblastoma Cell Line
2
Neuroblastoma Cell Culture and Analysis
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Human neuroblastoma cells line SK-N-SH (ATCC® HTB11™) were purchased from American Type Culture Collection (ATCC, USA). Minimum essential medium (MEM), fetal bovine serum (FBS), penicillin-streptomycin, Trypsin, and phosphate buffered saline (PBS) solutions were obtained from Gibco Life Technologies (Invitrogen, USA). Dimethyl sulfoxides (DMSO), retinoic acid (RA), thiazolyl blue tetrazolium bromide (MTT), and forskolin were purchased from Sigma-Aldrich (USA). Morphine sulphate pentahydrate (M-35-SU) and d,I-methadone.HCl (MET-637) were purchased from Lipomed AG (Switzerland). Isobutylmethylxanthine (IBMX) and radioimmunoprecipitation assay (RIPA) buffer and protease inhibitor were purchased from Amresco, USA. The antibodies used, α-synuclein, calmodulin, synaptotagmin 1, VAMP 2, anti-β-actin, and horseradish peroxidase (HRP) were purchased from Cell Signaling Technology (Massachusetts). WesternBright™ ECL and WesternBright Peroxide were purchased from Advansta (USA).
3
Culturing HTB-11 Neuroblastoma Cells
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HTB-11 human neuroblastoma cells (SK-N-SH [ATCC ® HTB-11™]) were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured in 10 mL of minimal essential media (Sigma-Aldrich, St. Louis, MO, USA) and supplemented with 10% fetal bovine serum in a 75 mL culture flask at 37°C and 5% CO 2 . Trypsin was then added to dissociate cell types. Post-trypsin dissociation, cells were transferred to 15-mL sterile Falcon tubes and centrifuged for 5 min at 1,000 rpm. The supernatant was discarded and the cell pellet fraction was collected and placed in 10 mL fresh culture medium for further incubation until reaching 100% confluence. Prior to each experiment, HTB-11 cells were counted and subcultured in a 96-well plate and incubated for 24 h.
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